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Pulsed-field gel electrophoresis as a molecular tool for characterizing genomes of certain food-borne bacterial isolates - a review

100%

Pal P.

International Letters of Natural Sciences

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2015

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tom 02

The evolutionary transition from phenotypic to molecular analysis of infectious disease in bacterial epidemiology led to the search for suitable approaches to ascertain genomic relatedness or heterogeneity between bacterial clinical isolates. Pulsed-field gel electrophoresis (PFGE) technique was developed for separating and analyzing long DNA fragments of several megabases in alternating electric field. Comparison of electrophoresis profiles of restriction enzyme-digested genomic DNA from bacterial isolates has proved to be a useful epidemiological tool for genetic discrimination of bacterial strains, detection of genetic relatedness, to locate the source of outbreak and to monitor the spread of the microorganisms in endemic zones. PFGE is considered as a gold standard method for typing of bacterial isolates because of the remarkable endurance of this technique as a typing method for the last 20 years in molecular epidemiology. In this current review the pros and cons of PFGE use in current molecular microbiological research are explored in the context of determination of genome organization of certain food-borne bacterial isolates causing infectious diseases in human beings.

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Identification of early and late replicating heterochromatic regions on platyfish [Xiphophorus maculatus] chromosomes

100%

Ocalewicz K.

Folia Biologica

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2005

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tom 53

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nr 3-4

3

Improvement of the strain for the rapid identification of genes encoding restriction and modification enzymes

100%

Piekarowicz A., Weglenska A.

Acta Microbiologica Polonica

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1994

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tom 43

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nr 2

4

Artificially engineered specific nucleases - a breakthrough in RE research - Review

100%

Cesnaviciene E.

Biotechnologia

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2003

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nr 2

5

In situ DNA targeting by restriction: a critical evaluation

100%

Olszewska M. J.

Prace Naukowe Uniwersytetu Śląskiego w Katowicach

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1998

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tom 1696

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Identification of a new restriction endonuclease R.BcrAI, from Bacillus cremoris

100%

Piekarowicz A., Skowronek K.

Acta Microbiologica Polonica

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1995

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tom 44

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nr 3-4

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PCR-RFLP-based molecular studies on inter-population variability in the crucian carp (Carassius carassius L.)

84%

Kempter J., Panicz R., Makowska M., Metza M., Keszka S., Kielpinski M., Sadowski J.

Zeszyty Naukowe. Acta Biologica. Uniwersytet Szczeciński

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2011

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tom 18

Genetic variability of three sympatric crucian carp ( Carassius carassius ) populations from NW Poland was studied within a research project aimed at assessing the utility of those populations for stocking in inland waters. DNA samples were collected from 65 individuals. Restriction analysis was performed using 4 enzymes (HaeIII, HinfI, FspBI, TasI) of known restriction sites. The restriction pro files obtained were classified as belonging to a single haplotype (H-1). Selected DNA products were sequenced; the subsequent comparison made it possible to detect the presence of substi- tutions in the genome fragment analysed.

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Restriction enzyme buffers and 5-azacytidine induce loss of protein and banding in plant metaphase chromosomes

84%

Olszewska M. J., Gernand D., Luchniak P., Sakowicz T.

Folia Histochemica et Cytobiologica

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1995

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tom 33

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nr 3

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Integration host factor [IHF] applied for partial digestion by restriction endonucleases in large DNA molecules [IARC procedure]

84%

Kur J.

Acta Microbiologica Polonica

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1993

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tom 42

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nr 2

10

Determination of genome size of Haemophilus influenzae Sb: Analysis of DNA restriction fragments

84%

Kauc L.

Acta Microbiologica Polonica

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1992

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tom 41

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nr 1-2

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Detection of DNA methylation in eucaryotic cells

67%

Sulewska A., Niklinska W., Kozlowski M., Minarowski L., Niklinski J., Dabrowska K., Chyczewski L.

Folia Histochemica et Cytobiologica

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2007

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tom 45

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nr 4

315-324

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Identyfikacja puroindolinowych alleli w krajowych odmianach pszenicy przy zastosowaniu markerow molekularnych

67%

Langner M., Salmanowicz B. P.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

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2009

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nr 253

93-101

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Use of IHF - mediated Achilles' heel cleavage [IHF-AC] method for mapping ihf sites

67%

Kur J.

Acta Microbiologica Polonica

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1993

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tom 42

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nr 2

14

Molecular differentiation of Entamoeba histolytica and Entamoeba dispar from stool and culture samples obtained from Polish citizens infected in Tropics and in Poland

67%

Myjak P., Kur J., Pietkiewicz H., Kotlowski A., Nahorski W., Szostakowska B.

Acta Protozoologica

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2000

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tom 39

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nr 3

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Length of restriction fragments is a factor determining DNA loss from metaphase chromosomes of Vicia faba

67%

Gernand D., Sakowicz T.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1992

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tom 40

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nr 2

16

Chromosome banding studies by replication and restriction enzyme treatment in vendace [Coregonus albula] [Salmonidae, Salmoniformes]

67%

Jankun M., Ocalewicz K., Mochol M.

Folia Biologica

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2004

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tom 52

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nr 1-2

Ograniczanie wyników

Pulsed-field gel electrophoresis as a molecular tool for characterizing genomes of certain food-borne bacterial isolates - a review

Identification of early and late replicating heterochromatic regions on platyfish [Xiphophorus maculatus] chromosomes

Improvement of the strain for the rapid identification of genes encoding restriction and modification enzymes

Artificially engineered specific nucleases - a breakthrough in RE research - Review

In situ DNA targeting by restriction: a critical evaluation

Identification of a new restriction endonuclease R.BcrAI, from Bacillus cremoris

PCR-RFLP-based molecular studies on inter-population variability in the crucian carp (Carassius carassius L.)

Restriction enzyme buffers and 5-azacytidine induce loss of protein and banding in plant metaphase chromosomes

Integration host factor [IHF] applied for partial digestion by restriction endonucleases in large DNA molecules [IARC procedure]

Determination of genome size of Haemophilus influenzae Sb: Analysis of DNA restriction fragments

Detection of DNA methylation in eucaryotic cells

Identyfikacja puroindolinowych alleli w krajowych odmianach pszenicy przy zastosowaniu markerow molekularnych

Use of IHF - mediated Achilles' heel cleavage [IHF-AC] method for mapping ihf sites

Molecular differentiation of Entamoeba histolytica and Entamoeba dispar from stool and culture samples obtained from Polish citizens infected in Tropics and in Poland

Length of restriction fragments is a factor determining DNA loss from metaphase chromosomes of Vicia faba

Chromosome banding studies by replication and restriction enzyme treatment in vendace [Coregonus albula] [Salmonidae, Salmoniformes]