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This study presents results of research on occurrence of chitinolytic bacteria and fungi in water, bottom sediments, and watershed soil of an eutrophic lake and on their ability to use the crustacean skeletons (shrimp waste) as a respiration substrate. It was found that the respiration rate of bacteria and fungi during decomposition of chitin varied in different environments. The participation of chitinolytic microorganisms in water (13%) and soil (18%) was greater than in bottom sediments (5%). The respiration activity in the presence of all parts of shrimp waste and shrimp exoskeletons observed in chitinolytic bacteria was higher than that of fungi. But fungi demonstrated the highest metabolic activity in the presence of the shrimp head sections. The highest respiration activity was observed in planktonic and soil bacteria, while the lowest, in benthic strains. The chitinolytic bacteria used well all examined respiration substrates (all parts of shrimp waste – 671 mg O₂ r⁻¹ protein in 5 days, the shrimp head sections – 851 mg O₂ r⁻¹ protein in 5 days and shrimp exoskeletons – 490 mg O₂ r⁻¹ protein in 5 days). No significant differences in respiration activity were observed in chitinolytic fungi isolated from water, bottom sediments and soil. All of fungal strains demonstrated the highest metabolic activity in the presence of the shrimp head sections (average 1083 mg O₂ r⁻¹ protein in 5 days). Shrimp exoskeletons were oxidized the least efficiently (average 160 mg O₂ r⁻¹ protein in 5 days). Certain strains were not using them at all.
This study investigated the effects of systemic alcohol injections on respiratory activity and short-term potentiation (STP) of the phrenic nerve and hypoglossal nerve activities, evoked by electrical stimulation of the superior laryngeal nerve (SLN), in anesthetized, paralyzed, and artificially ventilated rabbits. Alcohol, in a dose of 500 mg/kg, given singly or in cumulative fractions of 100mg/kg, depressed hypoglossal activity with little or no effect on phrenic activity. SLN stimulation inhibited both phrenic and hypoglossal activities and this effect remained unchanged by either way of alcohol administration. After cessation of stimulation, hypoglossal activity increased above the control level and slowly declined to the baseline, showing signs of STP. The amplitude and duration of the hypoglossal STP decreased following a single dose of alcohol. Cumulative fractions of the alcohol dose evoked a biphasic effect on the respiratory STP. In a lower range, alcohol enhanced the hypoglossal STP and tended to increase the duration of the phrenic STP. This effect gradually declined with increasing cumulative dose of alcohol and finally reversed to the inhibition of the STP of both nerves. The results demonstrate a dose-dependent biphasic effect of alcohol on the induction and maintaining of the hypoglossal STP. A reduction in STP, together with hypoglossal activity depression following alcohol accumulation, may contribute to the facilitation of upper airway obstruction by alcohol.
From March 1997 to March 1998 the photosynthesis (PHS) and respiration of seston (Rs) and bacteria fraction (Rb) in the water of the Upper Vistula River on the section from 10.9 to 336.7 km of the river course were investigated Methods applied: PhS - light and dark bottles, Rs - dark bottles. Rb - respiration of bacterial fraction, which was isolated by water filtration. The smallest mean yearly value of PhS (2.1-8.6 J dm⁻³ 24 h⁻¹) was found at 10.9-36.6 km of the river course and the greatest ones (40.4-42.7 J dm⁻³ 24 h⁻¹) at 248.2-336.7 km, in accordance with the size of the river. Rs was the smallest (23.9-28.7 J dm⁻³ 24 h⁻¹) between 10.9-45.7 km of the river course (on the rather clean sector) and decisively greater (51.6 J dm⁻³ 24 h⁻¹) at 115.6 km, at the most polluted sites. Along longitudinal profile of the Upper Vistula River Rb was equalized and ranged from 20.7 to 24.1 J dm⁻³ 24 h⁻¹, with the exception at 10.9 (spring, clean part of the river, poor in seston) and 336.7 km of the river course, where it was 10-20% smaller. The last value might indicated the presence of organic matter easily available to bacteria at this site. Rb constituted from 45 to 78% of seston Rs with the minimal value at 115.6 km of the river course (the most polluted) and its maximal participation at 10.9 km - clean part of the river, rather poor in seston.
The effect of different concentrations of mercury on the number and respiratory activity of neustonic and planktonic bacteria derived from estuarine Lake Gardno (Baltic Coast, Poland) was studied. The laboratory experiments demonstrated that mercuric ions (Hg²⁺) exhibited toxic effects on abundance and oxygen uptake of neustonic and planktonic bacteria. Bacterioneuston and bacterioplankton showed different responses to the mercuric ions. The neustonic bacteria showed higher levels of tolerance to various concentrations of Hg (10–15 mg dm⁻³) in the culture medium than the planktonic ones (<10 mg dm⁻³). It was found out that the bacteria isolated from freshwater zone of Lake Gardno were more tolerant to the increasing concentration of mercury ions (10–20 mg dm⁻³) than bacteria isolated from the seawater and mixed zones of lakes. Non-pigmented bacteria from all layers and zones were more torelant to the higher Hg²⁺ concentrations than pigmented ones. High concentrations (above 8 µg cm⁻³) of mercury chloride in the respiratory substrate blocked completely oxygen uptake by neustonic and planktonic bacteria.
In the bacterial community occurring in a sandy marine beach (region of the Gdańsk Gulf, Southern Baltic Sea), bacteria of the genera Acinetobacter and Microoccocus predominated among 230 isolated strains. Bacteria strains of the genera Alteromonas, Bacillus, Cytophaga, Erwinia and Prostheocomicrobium contributed in a small percent. The measurements of respiratory activity revealed that casein hydrolyzate was the most actively metabolised respiratory substrate while sodium pyruvate and cellobiose were oxidised less actively. The intensity of utilization of respiratory substrates by bacteria in the whole perpendicular profile of the beach was alike. They were more intensive in the surface (0–1 cm) than in the subsurface (5–10 cm) sand layers.
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