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Laboratory experiments were conducted to determine the effect of Silicosec by using contact and oral bioassay methods on survival of 1-7 days old adults of Oryzaeohilus surinamensis (L.) and Tribolium castaneum (Herbst) at 27±2°C and 65±5% RH. For contact method adult of these species were exposed to five doses of Silicosec for 3 days on plastic Petri dishes while in the case of oral bioassay technique the species were exposed to maize treated with five doses of Silicosec in small glass vials for 10 days. After exposure the initial mortality was recorded. For O. surinamensis in oral and contact methods 99% mortality was achieved at 8 333 ppm after 10 days and 165 ppm after 3 days, respectively, for T. castaneum 75% mortality achieved at 25 000 ppm after 10 days and 165 ppm after 3 days in the same order. For the oral method LC50 values for O. surinamensis and T. castaneum were 50 and 133 ppm, respectively after 10 days, while for the contact method these criteria were 8 and 57 ppm, respectively after 3 days. The results revealed that Silicosec with contact method could have more deleterious effect compared to the other method, and O. surinamensis was more susceptible to Silicosec than T. castaneum in either bioassay methods.
Humans and animals come into contact with various compounds in their natural environment. Most of the encountered substances are neutral, yet some may carry adverse health effects. The ingested food may be a source of harmful substances, including benzoquinones which, as shown by research results cited in this paper, demonstrate toxic, carcinogenic and enterotoxic activity. This group of compounds is inclusive of 2-methyl-1,4-benzoquinone (MBQ) and 2-ethyl-1,4-benzoquinone (EBQ), defensive secretions of the confused flour beetle (Tribolium confusum J. du V) and the red flour beetle (Tribolium castaneum Herbst). Benzoquinones have a carcinogenic effect, they are inhibitors of growth of various microorganisms, they produce a self-defense mechanism in threat situations and affect population aggregation. As noted by the referenced authors, the properties of benzoquinones have not been fully researched to this date.
Study on the sterilizing and lethal effects of Cobalt-60 gamma radiation on the larval and adult stages of rust red flour beetle Tribolium castaneum (Herbst.) was done at 0, 10, 30, 50, 70, 100, 150, 300 and 500 gray (Gy). Irradiation severely affected pupal period, pupal formation and survivability of larvae and adults. The developmental period of the pest was found to increase along with the increase in the radiation doses. The irradiation at 70 Gy was found to cause complete sterility in the insect.
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