The results of studies showed that residues of 2,4-D bound in the soil are available for wheat plants. It was found that most of radioactive residues detected in the soil were accumulated in fulvic acids fraction soluble in the water. Therefore, this part of residues can be available not only for progressive plants, but also for soil and aquatic organisms. The binding of 2,4-D residues with the soil matter turned out to be a partly reversible process, which progressed under biotic as well as abiotic factors. The soil microorganisms play a beneficial role in the soil environment. Due to their activity the bound residues are released in small amounts into soil solution, and mineralized more than under abiotic factors.