Wyniki wyszukiwania

1

The search for QTL in barley (Hordeum vulgare L.) using a new mapping population

100%

Buck-Sorlin G. H.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr 2A

Ninety-nine different lines of the Doubled-Haploid F2 winter barley population W766 ('Angora’ x 'W704/137') were genetically fingerprinted using AFLP, microsatellite, morphological and resistance markers. A preliminary map consisting of seven linkage groups is presented. The map contains a highly distorted region on the long arm of chromosome 3H reflecting preselection of the genotypes for resistance against barley mild mosaic virus. QTL analysis of morphological and phenological traits yielded 99 significant QTL, with most traits (66.3%) being represented by a single QTL. The distribution of significant QTL over the chromosomes was very uneven, the bulk being placed on the long arm of chromosome 3H and no QTL being found on chromosome 4H. This possibly points to the presence of a strong pleiotropic gene on 3H or of a group of related genes that mask weaker effects that were found on other linkage groups as subsignificant QTL. Using two examples of detected QTL (for tillering and grain number), it is shown how the findings of the QTL analysis could be incorporated into an existing morphological simulation model of barley using simple statistical methods.

2

The identification of QTLs associated with the in vitro response of rye [Secale cereale L.]

86%

Bolibok H., Gruszczynska A., Hromada-Judycka A., Rakoczy-Trojanowska M.

Cellular and Molecular Biology Letters

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2007

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tom 12

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nr 4

523-535

This study was conducted in order to identify quantitative trait loci (QTLs) for the in vitro culture response of winter rye (Secale cereale L.) immature embryos and immature inflorescences. A genetic linkage map comprising 67 SSRs, 9 ISSRs, 13 SAMPLs, 7 RAPDs, 2 SCARs and one EST marker was created based on the analyses of 102 recombinant inbred lines from the cross between lines L318 (which has a good response in tissue cultures) and L9 (which is unable to regenerate plants from somatic tissues and anthers). The map spans 979.2 cM, and the average distance between markers is 9.9 cM. Two characteristics were evaluated: callus induction (CI) and somatic embryogenesis ability (SE). They were expressed as the percentage of immature embryos/inflorescences producing callus (designated ECI/ICI) and the percentage of explants producing somatic embryos (ESE/ISE). All the analysed traits showed continuous variation in the mapping population but a non-normal frequency distribution. We identified nine putative QTLs controlling the tissue culture response of rye, explaining up to 41.6% of the total phenotypic variation: two QTLs for ECI — eci-1, eci-2; 4 for ESE — ece-1, ese-2, ese-3, ese-4; 2 for ICI — ici-1, ici2; and 1 for ISE — ise-1. They were detected on chromosomes 1R, 4R, 5R, 6R and 7R.

3

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Characterization and mapping of QTL used in breeding of Scots pine (Pinus sylvestris L.)

86%

Nowicka A., Ukalska J., Siminska J., Szyp-Borowska I.

Folia Forestalia Polonica. Series A . Forestry

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2013

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tom 55

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nr 4

This paper reports the construction a map based on Amplified Fragment Length Polymorphic DNA (AFLP) in Scots pine (Pinus sylvestris L.). The main purpose of map construction was its application to quantitative traits loci (QTL) mapping for breeding traits economically important in Scots pine breeding program such as tree height and diameter at breast height, number of needles and their length, width, and area. Genomic DNA of needles and haploid megagamethophytes from seeds originating from a single tree were amplified with 25 AFLP primer-enzyme combinations with three or four selective nucleotides. Sixteen of them generated easily readable patterns and revealed a polymorphism. Each analyzed marker was tested for the expected 1 : 1 segregation ratio using χ2 – test and only 6 were significant with (α ≤ 0.05). The total map size equaled 291,7 cM and all markers were distributed within one linkage group. For all traits only one QTL associated with tree height (H) was detected.

4

Application of bioinformatics in fruit plant breeding

86%

Vassilev D., Nenov A., Atanassov A., Dimov G., Getov L.

Journal of Fruit and Ornamental Plant Research

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2006

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tom 14

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nr Suppl.1

5

Natural variation in ozone induced cell death in Arabidopsis

72%

Leppala J., Brosche M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

|

nr 2

6

Identification of novel genes potentially involved in rice (Oryza sativa L.) drought tolerance

72%

Zinati Z.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2017

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tom 98

|

nr 3

7

The mapping of QTLs for chlorophyll content and responsiveness to gibberellic (GA3) and abscisic (ABA) acids in rye

72%

Milczarski P., Masojc P.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr 2A

Genetic maps containing molecular markers are useful tools for the identification of genes underlying quantitative traits (QTLs). Three traits important for plant physiology, i.e. chlorophyll content, sensitivity to GA and sensitivity to ABA, were evaluated for 99 F2 families of the DS2 x RXLl0 rye mapping population. The observed variation in the chlorophyll content in rye leaves was shown to be under the genetic control of four independent QTLs. They were located on the following chromosome arms: 1RL (QChc-1R.I), 3RS (QChc-3R.1), 4RL (QChc-4R.1) and 5RL (QChc-5R.1) The marker-assisted selection of recombinants comprising positively-acting alleles at these loci may significantly increase the chlorophyll content in rye leaves, which should result in a higher efficiency of photosynthesis. A decreasing of plant height in rye is one of the major aims of breeders selecting for lodging resistance. The polymorphism of genes controlling sensitivity to gibberellic acid may be the basis for a potential selection strategy. Three QTLs underlying the sensitivity of rye seedlings to GA were located on chromosomes 5RL (QGar-5R.I), 1RL (QGar-1R.1) and on 7RL (QGar-7R.I). The dwarfing allele of a Dw1 locus present in line RXL10 was mapped in the same position as the QGar-5R.1 gene. Sensitivity to ABA plays an important role in imposing dormancy in rye grain. Three QTLs affecting sensitivity to ABA were found. They were mapped on chromosomes 1RS (QAbr-1R.1), 2R in the centromeric region (QAbr-2R.1) and on 5RL (QAbr-5R.1). QAbr-5R.1, Dw1 and QGar-5R.1 are located in the same position on the distal part of the 5RL chromosome arm, which suggests that they belong to a single locus for a major regulatory gene controlling the development of rye plants.

8

High-throughput phenotyping and genotyping in comparative QTL analysis of early short-time drought tolerance in Polish fodder and malting spring barleys

72%

Wojcik-Jagla M., Rapacz M., Tyrka M., Koscielniak J., Crissy K., Zmuda K.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 3

9

Identyfikacja QTL warunkujacych dlugosc liscia flagowego w dwoch populacjach mapujacych zyta [Secale cereale L.]

72%

Milczarski P.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

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2008

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nr 250

203-209

10

Identification of QTL for maize resistance to common smut by using recombinant inbred lines developed from the Chinese hybrid Yuyu22

72%

Ding J. Q., Wang X. M., Chander S., Li J. S.

Journal of Applied Genetics

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2008

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tom 49

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nr 2

147-154

Common smut in maize, caused by Ustilago maydis, reduces grain yield greatly. Agronomic and chemical approaches to control such diseases are often impractical or ineffective. Resistance breeding could be an efficient approach to minimize the losses caused by common smut. In this study, quantitative trait loci (QTL) for resistance to common smut in maize were identified. In 2005, a recombinant inbred line (RIL) population along with the resistant (Zong 3) and susceptible (87-1) parents were planted in Beijing and Zhengzhou. Significant genotypic variation in resistance to common smut was observed at both locations after artificial inoculation by injecting inoculum into the whorl of plants with a modified hog vaccinator. Basing on a genetic map containing 246 polymorphic SSR markers with an average linkage distance of 9.11 cM, resistance QTL were analysed by composite interval mapping. Six additive-effect QTL associated with resistance to common smut were identified on chromosomes 3 (three QTL), 5 (one QTL) and 8 (two QTL), and explained 3.2% to 12.4% of the phenotypic variation. Among the 6 QTL, 4 showed significant QTL x environment (Q x E) interaction effects, which accounted for 1.2% to 2.5% of the phenotypic variation. Nine pairs of epistatic interactions were also detected, involving 18 loci distributed on all chromosomes except 2, 6 and 10, which contributed 0.8% to 3.0% of the observed phenotypic variation. However, no significant epistasis x environment interactions were detected. In total, additive QTL effects and Q x E interactions explained 38.8% and 8.0% of the phenotypic variation, respectively. Epistatic effects contributed 15% of the phenotypic variation. The results showed that besides the additive QTL, both epistasis and Q x E interactions formed an important genetic basis for the resistance to Ustilago maydis in maize.

11

Cross-species bacterial artificial chromosome [BAC] library screening via overgo-based hybridization and BAC-contig mapping of a yield enhancement quantitative trait locus [QTL] YLD1.1 in the Malaysian wild rice Oryza rufipogon

72%

Song B. K., Nadarajah K., Romanov M. N., Ratnam W.

Cellular and Molecular Biology Letters

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2005

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tom 10

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nr 3

12

Bovine kappa-casein [CASK] gene - molecular nature and application in dairy cattle breeding

72%

Kaminski S.

Journal of Applied Genetics

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1996

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tom 37

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nr 2

The bovine kappa-casein (CASK) gene is considered a potential marker for quantitative trait loci (QTL) in dairy cattle. A large amount of research has been performed to explore the nature and variation of the CASK gene and its possible applications in cattle breeding. The purpose of this review is to sum up the knowledge of all known aspects of the CASK gene: molecular structure and function, polymorphism and allele freqeuncy, methods of genotyping and possibilities of the use of CASK polymorphism in dairy cattle breeding.

13

Genetic associations of reproductive traits in pigs

58%

Kumalska M., Terman A.

Annals of Warsaw University of Life Sciences - SGGW. Animal Science

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2017

|

tom 56[2]

Genetic associations of reproductive traits in pigs. In the field of genetics, one of the main research area in relation to animal reproduction is the identification of genes or genomic regions influencing reproductive phenotypes. The genes analysed for the determinants of their fertility are among other: LEP, PRL, PRLR, RBP4. With the use of genetic markers, it is possible to identify of both males and females carrying beneficial alleles, and choose reproduce high-quality individuals, which in turn accelerates the genetic improvement of the examined feature. According to literature, about 30% of culling in pig production systems has been primarily due to reproductive problems. Litter size is very important and easily measured reproductive trait, and often included in scientific researches, and defined as the total number of piglets born (TNB) and the number of piglets born alive (NBA). Selection of individuals carrying favourable alleles has the potential to improve reproductive traits and in this connection also sow productive life (SPL). SLP is a measure of the longevity and reproductive performance of a sow and is directly related to the number of viable piglets produced during its lifespan. Because reproductive traits are so multifaceted, researchers are able to consider many different facets of the organism biology to come up with candidate genes and QTLs genes.

14

Genetyka ilosciowa - przeglad przez stulecie

58%

Surma M., Adamski T., Kaczmarek Z., Krajewski P.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

|

2008

|

nr 250

5-19

15

Sposoby epistatycznego dzialania genow u zyta ozimego

58%

Smialowski T., Wegrzyn S.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

|

2007

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nr 244

173-181

16

Saturation and mapping of a major Fusarium head blight resistance QTL on chromosome 3BS of Sumai 3 wheat

58%

Zhou M.-P., Hayden M. J., Zhang Z.-Y., Lu W.-Z., Ma H.-X.

Journal of Applied Genetics

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2010

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tom 51

|

nr 1

19-25

Fusarium head blight (FHB) is a destructive disease in wheat. The major quantitative trait locus (QTL) on 3BS from Sumai 3 and its derivatives has been used as a major source of the resistance to FHB worldwide, but the discrepancy in reported location of the major QTL could block its using in map based cloning and marker assisted selection. In this study, Chinese Spring-Sumai 3 chromosome 3B substitution line was used as resistant parent of the mapping population to reduce the confounded effect of genetic background in Sumai 3. The major QTL region was saturated with the Sequence Tagged Microsatellite (STM) and Sequence Tagged Site (STS) markers. A linkage map of chromosome 3B with 36 markers covering a genetic distance of 112.4 cM was constructed. Twelve new markers were inserted into the chromosome region where the major QTL was located. The average interval distance between markers was 1.5 cM. Multiple QTL Models (MQM) mapping indicated that the major QTL was located in the interval of Xgwm533-Xsts9-1, and explained 45.6% of phenotypic variation of the resistance to FHB. The SSR (simple sequence repeat) marker Xgwm533 and STM marker Xstm748tcac are closely linked to the major QTL.

17

Mutations in the bovine ABCG2 and the ovine MSTN gene added to the few quantitative trait nucleotides identified in farm animals: a mini-review

51%

Braunschweig M. H.

Journal of Applied Genetics

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2010

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tom 51

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nr 3

The progress in molecular genetics in animal breeding is moderately effective as compared to traditional animal breeding using quantitative genetic approaches. There is an extensive disparity between the number of reported quantitative trait loci (QTLs) and their linked genetic variations in cattle, pig, and chicken. The identification of causative mutations affecting quantitative traits is still very challenging and hampered by the cloudy relationship between genotype and phenotype. There are relatively few reports in which a successful identification of a causative mutation for an animal production trait was demonstrated. The examples that have attracted considerable attention from the animal breeding community are briefly summarized and presented in a table. In this mini-review, the recent progress in mapping quantitative trait nucleotides (QTNs) are reviewed, including the ABCG2 gene mutation that underlies a QTL for fat and protein content and the ovine MSTN gene mutation that causes muscular hypertrophy in Texel sheep. It is concluded that the pi ogress in molecular genetics might facilitate the elucidation of the genetic architecture of QTLs, so that also the high-hanging fruits can be harvested in order to contribute to efficient and sustainable animal production.

18

Przydatnosc markera QTL w hodowli jabloni odpornej na maczniaka [Podosphaera leucotricha [Ellis et Ev.] Salm.]

51%

Stankiewicz-Kosyl M., Pitera E., Gawronski S. W.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

|

2006

|

nr 242

295-301

19

Rozwazania nad mapowaniem genetycznym QTL odpowiedzialnym za ceche zoltonasiennosci rzepaku ozimego [Brassica napus L.]

51%

Hernacki B., Bartkowiak-Broda I., Piotrowska A., Cegielska-Taras T.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

|

2009

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nr 253

221-229

20

Identyfikacja QTLs cech uzytkowosci mlecznej bydla metoda skanowania genomu i analizy sprzezen genetycznych zgodnie z procedura "selective DNA pooling"

51%

Walawski K., Pareek C. S., Zabolewicz T., Czarnik U.

Biuletyn Informacyjny. Instytut Zootechniki

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2002

|

tom 40

|

nr 2

75-90

Ograniczanie wyników

The search for QTL in barley (Hordeum vulgare L.) using a new mapping population

The identification of QTLs associated with the in vitro response of rye [Secale cereale L.]

Characterization and mapping of QTL used in breeding of Scots pine (Pinus sylvestris L.)

Application of bioinformatics in fruit plant breeding

Natural variation in ozone induced cell death in Arabidopsis

Identification of novel genes potentially involved in rice (Oryza sativa L.) drought tolerance

The mapping of QTLs for chlorophyll content and responsiveness to gibberellic (GA3) and abscisic (ABA) acids in rye

High-throughput phenotyping and genotyping in comparative QTL analysis of early short-time drought tolerance in Polish fodder and malting spring barleys

Identyfikacja QTL warunkujacych dlugosc liscia flagowego w dwoch populacjach mapujacych zyta [Secale cereale L.]

Identification of QTL for maize resistance to common smut by using recombinant inbred lines developed from the Chinese hybrid Yuyu22

Cross-species bacterial artificial chromosome [BAC] library screening via overgo-based hybridization and BAC-contig mapping of a yield enhancement quantitative trait locus [QTL] YLD1.1 in the Malaysian wild rice Oryza rufipogon

Bovine kappa-casein [CASK] gene - molecular nature and application in dairy cattle breeding

Genetic associations of reproductive traits in pigs

Genetyka ilosciowa - przeglad przez stulecie

Sposoby epistatycznego dzialania genow u zyta ozimego

Saturation and mapping of a major Fusarium head blight resistance QTL on chromosome 3BS of Sumai 3 wheat

Mutations in the bovine ABCG2 and the ovine MSTN gene added to the few quantitative trait nucleotides identified in farm animals: a mini-review

Przydatnosc markera QTL w hodowli jabloni odpornej na maczniaka [Podosphaera leucotricha [Ellis et Ev.] Salm.]

Rozwazania nad mapowaniem genetycznym QTL odpowiedzialnym za ceche zoltonasiennosci rzepaku ozimego [Brassica napus L.]

Identyfikacja QTLs cech uzytkowosci mlecznej bydla metoda skanowania genomu i analizy sprzezen genetycznych zgodnie z procedura "selective DNA pooling"