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1

Relationship between the molecular typing of Campylobacter strains and the prevalence of their virulence genes

100%
Wieczorek K.
Bulletin of the Veterinary Institute in Puławy
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2009
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tom 53
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nr 2
193-198
The aim of this study was to evaluate and compare two genotyping methods for the examination of genetic diversity in C. jejuni and C. coli strains isolated from poultry. Moreover, the molecular identification of putative virulence factors was additionally included to establish a correlation between the isolates. Both typing methods, RAPD-PCR and ERIC-PCR, were found to have a high discriminatory power, D=0.95 and 0.94, respectively. The isolates were clustered to 28 groups by RAPD-PCR and to 29 by ERIC- PCR. Sometimes the ERIC or RAPD assays were able to further discriminate the isolates, which had the same virulence profile generated by the second technique. These PCR-based differentiation methods demonstrated the potential for initial rapid epidemiology studies, and when used in conjunction with distribution of virulence markers provide a high resolution strategy for accurately defining subtypes of Campylobacter.
2
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Enhanced expression of mineralocorticoid receptors in the heart after the myocardial infarct in rats

80%
Milik E., Szczepanska-Sadowska E., Maslinski W., Cudnoch-Jedrzejewska A.
Journal of Physiology and Pharmacology
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2007
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tom 58
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nr 4
Increasing evidence suggests that enhanced stimulation of the heart and kidney by mineralocorticoids plays significant role in development of the post-infarct cardiac failure. Because increased synthesis of mineralocorticoid receptors (MR) is one of the putative factors determining pathogenic effects of mineralocorticoids we decided to determine whether the myocardial infarct results in an enhanced expression of MR mRNA and MR protein. To this end male Sprague-Dawley rats were subjected either to ligation of the left coronary artery or to sham surgery. After four weeks expressions of MR mRNA and MR protein were evaluated in both groups of rats in the left (LV) and right (RV) ventricle walls, and in the renal cortex and renal medulla by means of semiquantitative PCR and Western blotting methods. Coronary ligation resulted in the myocardial infarction encompassing 30.2% ± 1.9% (range 23-40%) of the left ventricle wall. In the infarcted rats expression of MR mRNA was significantly greater than in the sham-operated rats, both in the LV (P<0.02) and in the RV (P<0.005). In the left but not in the right ventricle increased MR mRNA expression was associated with significant increase in expression of MR protein (P<0.001). In the renal cortex and renal medulla MR mRNA and MR protein expression in the infarcted and the sham-operated rats did not differ. The study reveals that during the post-infarct state expression of MR mRNA is elevated in both cardiac ventricles while expression of MR mRNA protein is increased only in the left ventricle. The results suggest that the enhanced expression of mineralocorticoid receptors may contribute to enhanced effects of mineralocorticoids in the heart during the post-infarct state.
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