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Przeciwciala dla Salmonella enteritidis w surowicy, zoltku i tkance miesniowej ptakow wykrywane metoda ELISA

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Rzedzicki J., Pilaszek J., Tokarzewski S., Szulowski K., Iwaniak W.

Medycyna Weterynaryjna

2000

tom 56

nr 04

235-239

Indirect ELISA is being used increasingly more often in serological diagnosis of Salmonella enteritidis in poultry. Tests are conducted on sera as well as to detect anti-S. enteritidis antibodies in egg yolk and in muscles. The aim of this study was to assess the usefulness of an ELISA kit produced in the authors’ laboratory in detecting anti-S. enteritidis antibodies in sera, egg yolk and muscles from poultry. The lipopolisaccharide (LPS) antigen obtained from the S. enteritidis phage type 1 strain was used in the kit as well as anti-chicken IgG conjugated to horseradish peroxidase and ARTS which was used as the substrate. Sera, egg yolk and meat juice were tested in a flock of hens suspected to be infected by Salmonella. The results obtained showed that some egg yolk and meat juice samples had low values of absorbency in ELISA, whereas others had high values. A high degree of correlation was obtained between the results of the tests carried out on egg yolk, meat juice and sera. The results of the investigations indicate that the above devised ELISA test based on LPS was useful for testing sera, egg yolk and muscles from poultry for the presence of anti-S.enteritidis antibodies.

Ograniczanie wyników

1 Medycyna Weterynaryjna

1 Iwaniak W.

1 Pilaszek J.

1 Rzedzicki J.

1 Szulowski K.

1 Tokarzewski S.

1 2000

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Przeciwciala dla Salmonella enteritidis w surowicy, zoltku i tkance miesniowej ptakow wykrywane metoda ELISA