The specificity of a panel containing 36 monoclonal antibodies (MAbs) for the diagnosis of swine fever (SF) was evaluated by the immunoperoxidase monolayer assay (IPMA). For the test were utilized microplates coated with the PK-15A cells which were infected with the following Hog cholera virus (HCV) strains: Alfort-187, TVM-1 and 10 field isolates. MDBK cells propagated on microplates were infected with Bovine viral diarrhoa virus (BVDV) of the NADL-158 strain. By means of polyclonal antibodies the presence of all of the HCV and BVDV strains which were bred were demonstrated. Nine of 36 examined MAbs reacted exclusively with Alfort-187 strain and with all field isolates. The above-mentioned antibodies did not form immuno- complexes with TVM-1 and NADL-158 strains. They were defined as specific antibodies to HCV. One MAb detected epitopes was present in the Alfort strain and in all but one field HCV isolates. This antibody did not detect the TVM-1 strain. Two clones specific for BVDV used in this study reacted exclusively with the NADL-158 strain. Twenty four supernatants collected from hybri- doma cell cultures produced distinct reactions on microplates coated with cells infected with the Alfort strain, field isolates and the NADL-158 strain. The above mentioned antibodies were defined as specific for the Pesti- virus genus. For the precise selection of MAbs, additional studies will be carried out, mainly in the range of evaluating MAbs with BVDV field strains.
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