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Paulinella chromatophora - rethinking the transition from endosymbiont to organelle

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Nowack E. C. M.

Acta Societatis Botanicorum Poloniae

2014

tom 83

nr 4

Eukaryotes co-opted photosynthetic carbon fixation from prokaryotes by engulfing a cyanobacterium and stably integrating it as a photosynthetic organelle (plastid) in a process known as primary endosymbiosis. The sheer complexity of interactions between a plastid and the surrounding cell that started to evolve over 1 billion years ago, make it challenging to reconstruct intermediate steps in organelle evolution by studying extant plastids. Recently, the photosynthetic amoeba Paulinella chromatophora was identified as a much sought-after intermediate stage in the evolution of a photosynthetic organelle. This article reviews the current knowledge on this unique organism. In particular it describes how the interplay of reductive genome evolution, gene transfers, and trafficking of host-encoded proteins into the cyanobacterial endosymbiont contributed to transform the symbiont into a nascent photosynthetic organelle. Together with recent results from various other endosymbiotic associations a picture emerges that lets the targeting of host-encoded proteins into bacterial endosymbionts appear as an early step in the establishment of an endosymbiotic relationship that enables the host to gain control over the endosymbiont.

The involvement of organelles in plant sexual reproduction: A post-genomic approach

100%

Berthome R., Froger N., Hiard S., Balasse H., Martin-Canadell A., Budar F.

Acta Biologica Cracoviensia. Series Botanica

2003

tom 45

nr 1

To study mitochondria and plastid involvement in plant development, particularly in sexual reproduction, we made use of the Arabidospis thaliana T-DNA insertion collection developed in Versailles. Mutants affected in the nuclear genes that encode proteins predicted to be targeted to organelles were identified using two complementary strategies. In the first (forward genetics), mutants chosen for their sterile or gametophytic lethal phenotype were screened for T-DNA insertion in genes encoding mitochondrial or plastid proteins after systematic sequencing of the Flanking Sequence Tag (FST). The second (reverse genetics) enabled us to identify other mutants using the following tools: systematic A. thaliana proteome analysis, bioinformatics software to predict the sub-cellular localization of putative proteins, and the FST sequencing program FlagDB. Preliminary results for the first set of 82 putative mutants are presented and discussed.

Ograniczanie wyników

1 Acta Biologica Cracoviensia. Series Botanica

1 Acta Societatis Botanicorum Poloniae

1 Balasse H.

1 Berthome R.

1 Budar F.

1 Froger N.

1 Hiard S.

1 Martin-Canadell A.

1 Nowack E. C. M.

1 2014

1 2003

Wyniki wyszukiwania

Paulinella chromatophora - rethinking the transition from endosymbiont to organelle

The involvement of organelles in plant sexual reproduction: A post-genomic approach