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Oligodeoxyadenylate stimulates the protein kinase activity of anti-DNA sIgA from human milk

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Kit Y., Kuligina E., Semenov D., Richter V.

Acta Biochimica Polonica

2002

tom 49

nr 1

Preparations of anti-DNA sIgA were obtained from human milk by sequential chromatography on protein A-sepharose, DEAE-fractogel and DNA-cellulose. The influence of oligonucleotides on protein kinase activity was investigated. It was discovered that incubation of anti-DNA sIgA with oligodeoxyriboadenylate d(A)12 stimulates the phosphorylation of polypeptides of sIgA in the presence of [γ- 32P]ATP. The greatest was the incorporation of 32P into the sIgA H-chains. We also demonstrated stimulation of the casein kinase activity of anti-DNA sIgA by d(A)12. The stimulation of the protein kinase activity of anti-DNA sIgA by oligoriboadenylate r(A)12 was not detected.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Kit Y.

1 Kuligina E.

1 Richter V.

1 Semenov D.

1 2002

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Oligodeoxyadenylate stimulates the protein kinase activity of anti-DNA sIgA from human milk