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The expression of genes coding for opioid precursors, opioids receptors, beta-LH subunit and GnRH receptor in the anterior pituitary of cyclic gilts

100%

Wylot B., Staszkiewicz J., Okrasa S.

Journal of Physiology and Pharmacology

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2008

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tom 59

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nr 4

745-758

In previous studies the effect of endogenous opioid peptides (EOP) on LH secretion was mainly considered at the hypothalamic level, while opioid involvement in the modulation of LH secretion at the pituitary level remains insufficiently elucidated. Therefore, the present study was undertaken to determine the expression of genes encoding opioid precursors – proopiomelanocortin (POMC), proenkephalin (PENK), prodynorphin (PDYN) and opioid receptors – mu, delta, kappa in the porcine anterior pituitary throughout the estrous cycle. Additionally, the mRNA content of ß-LH subunit and GnRH receptor (GnRH-R) was estimated. Pituitaries (5×N = 7) were collected from sows on days 3-5, 8-10, 13-15, 16-17 and 19-20 of the cycle and gene expression was determined using a semi-quantitative RT-PCR assay. The expression of POMC, PDYN, delta and kappa receptor genes was variable across the cycle, whereas the expression of PENK and mu receptor genes remained relatively stable. The POMC mRNA content was the lowest on days 19-20 of the cycle and the PDYN content was reduced on days 8-10. The delta receptor mRNA content was elevated on days 3-5, while the kappa receptor mRNA content was decreasing over the luteal phase. Changes in the expression of genes encoding ß-LH and GnRH-R were also demonstrated. These results indicate variable activity of pituitary opioid systems in cyclic pigs and suggest implication of EOP in the modulation of LH secretion at the pituitary level.

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Proopiomelanocortin but not vasopressin or renin-angiotensin system induces resuscitative effects of central 5-HT1A activation in haemorrhagic shock in rats

100%

Sowa P., Adamczyk-Sowa M., Zwirska-Korczala K., Pierzchala K., Adamczyk D., Paluch Z., Misiolek M.

Journal of Physiology and Pharmacology

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2014

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tom 65

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nr 5

3

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Modulation of testicular functions by testicular opioid peptides

80%

Gerendai I.

Journal of Physiology and Pharmacology

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1991

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tom 42

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nr 4

The possible physiological role of testicular opioid peptides in the control of testicular functions has been studied. In neonatal rats in tratest icular administration of opiate receptor antagonists (naloxone, nalmefene) stimulates Sertoli cell proliferation and secretion. Both in adult and neonatal rats local injection of the testis with opiate receptor antagonists or with ß-endorphin antiserum results in a decrease in steroidogenesis in long-term studies. Treatment of neonatal testis with an enkephalin analogue induces a short-term suppression of testosterone secretion. Further studies were carried out to investigate whether the above described local effects of opiate agonist or antagonist on testicular function are under the regulatory control of testicular nerves. Partial denervation of the testis was performed by testicular injection of 6-hydroxydopamine (a neurotoxin degenerating sympathetic neural structures) or by vasectomy (cutting the inferior spermatic nerve). If testicular administration of opioid agonist or antagonist was combined with partial denervation of the testis, the effects of pharmacological agents influencing testicular opioid level were not evident. The data indicate that opioid peptides synthesized in the testis are components,of the intratest icular regulatory system and that local opioid actions are modulated by testicular nerves.

Ograniczanie wyników

3 Journal of Physiology and Pharmacology

1 Adamczyk D.

1 Adamczyk-Sowa M.

1 Gerendai I.

1 Misiolek M.

1 Okrasa S.

1 Paluch Z.

1 Pierzchala K.

1 Sowa P.

1 Staszkiewicz J.

1 Wylot B.

1 Zwirska-Korczala K.

1 2014

1 2008

1 1991

The expression of genes coding for opioid precursors, opioids receptors, beta-LH subunit and GnRH receptor in the anterior pituitary of cyclic gilts

Proopiomelanocortin but not vasopressin or renin-angiotensin system induces resuscitative effects of central 5-HT1A activation in haemorrhagic shock in rats

Modulation of testicular functions by testicular opioid peptides