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An insertion-deletion polymorphism in the 3'UTR encoding region of the porcine prolactin [PRL] gene

100%

Babicz M., Pierzchala M., Urbanski P., Rozempolska-Rucinska I.

Animal Science Papers and Reports

2008

tom 26

nr 3

A novel mutation, insertion/deletion (InDel) was found in the porcine prolactin gene exon 5 coding for the 3’UTR. Polymorphism was detected and identified using PCR-MSSCP and DNA sequencing techniques, and then analysed with PCR-RFLP using HpyCH4III endonuclease. Inserted/deleted was an 11-bp TGTTTCTTAAC motive that occurred once or twice at position 201-222 in the amplified DNA fragment. The investigations covered 150 gilts of the Puławska breed from the herds under the Preserve Breeding Project. The frequencies of the inserted and deleted PRL gene variants were 0.34 and 0.66, respectively. The estimated value of heterozygosity (Ĥ) was 0.253 and the polymorphism information content (PIC) amounted to 0.348.

Novel SNP of the goat prolactin gene [PRL] associated with cashmere traits

84%

Lan X.-Y., Pan C.-Y., Chen H., Lei C.-Z., Li F.-Y., Zhang H.-Y., Ni Y.-S.

Journal of Applied Genetics

2009

tom 50

nr 1

51-54

Concentrations of the single-chain polypeptide hormone prolactin (PRL) are associated with wool or cashmere traits, and its seasonal changes may determine patterns of enzymatic activity and may affect cashmere fibre growth and moult. So, the PRL gene is a potential candidate gene for cashmere traits in marker-assisted selection (MAS). In this paper, we report a novel missense single-nucleotide polymorphism (SNP) within the goat PRL gene in 1367 individuals by PCR-SSCP (polymerase chain reaction with single-strand conformation polymorphism) analysis and DNA sequencing. The novel X76049:g.576C>A mutation is confirmed by Eco241 PCR-RFLP (restriction fragment length polymorphism) analysis and causes a missense codon (Pro176Thr). The frequencies of allele C varied from 0.79 to 0.93 in 9 analysed goat populations. C allele was correlated with higher fibre length (P = 0.014).

Nucleotide sequence polymorphism within exon 4 of the bovine prolactin gene and its associations with milk performance traits

67%

Brym P., Kaminski S., Wojcik E.

Journal of Applied Genetics

2005

tom 46

nr 2

Prolactin plays an important regulatory function in mammary gland development, milk secretion, and expression of milk protein genes. Hence the PRL gene is a potential quantitative trait locus and genetic marker of production traits in dairy cattle. We analysed the sequence of the PRL gene to investigate whether mutations in this sequence might be responsible for quantitative variations in milk yield and composition. Using SSCP and direct sequencing, we detected six single-nucleotide polymorphisms within a 294-bp prolactin gene fragment involving exon 4. All detected mutations were silent with respect to the amino acid sequence of the protein. PCR-RFLP genotyping of SNP 8398 R (Rsal) was used to assess allele frequencies in 186 Black-and-White cows (0.113 and 0.887 for A and G, respectively) and in 138 Jersey cows (0.706 and 0.294 for A and G, respectively). Black-and-White cows with genotype AG showed the highest milk yield, while cows with genotype GG showed the highest fat content.

Ograniczanie wyników

2 Journal of Applied Genetics

1 Animal Science Papers and Reports

1 Babicz M.

1 Brym P.

1 Chen H.

1 Kaminski S.

1 Lan X.-Y.

1 Lei C.-Z.

1 Li F.-Y.

1 Ni Y.-S.

1 Pan C.-Y.

1 Pierzchala M.

1 Rozempolska-Rucinska I.

1 Urbanski P.

1 Wojcik E.

1 Zhang H.-Y.

1 2009

1 2008

1 2005

Wyniki wyszukiwania

An insertion-deletion polymorphism in the 3'UTR encoding region of the porcine prolactin [PRL] gene

Novel SNP of the goat prolactin gene [PRL] associated with cashmere traits

Nucleotide sequence polymorphism within exon 4 of the bovine prolactin gene and its associations with milk performance traits