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The objectives of this study were to define the effect of the milk progesterone (P4) concentration on estrus expression in dairy cows with high milk yield and to identify the effect of milk yield and parity on the milk P4 concentration and cow’s pregnancy after artificial insemination (AI). In this study, 48 clinically healthy cows without reproduction disorders, on day 90-100 after calving were used. At the beginning of estrus and 12 hours after the beginning of estrus, the milk P4 concentration in dairy cows with high milk yield (group 3) was higher than in group 2 (33.66; 22.36%) and in group 1 (51.60; 65.26%) (P < 0.001). The milk P4 concentration in the ≥ 3 lactation cows was higher than the second (13.45%; P > 0.05) and the first (28.28%; P < 0.01) lactation ones (28.28%; P < 0.01). The milk P4 concentration at the beginning of estrus and 12 hours after the beginning of estrus in pregnant and non-pregnant cows was 2.58 and 3.32; 4.20 and 5.00 ng/ml, respectively (P < 0.001). As a result, it was concluded that high progesterone concentration affected the expression of estrus and pregnancy results in dairy cows, and the measuring of progesterone concentration in milk can be used as a non-invasive method to provide detailed information about fertility in high milk yield cows.
The experiment was carried out on nine sexually mature, aged 1-3 years, clinically healthy bitches being in anoestrus. The animals were divided into two experimental groups and one control group. The bitches from the experimental groups were receiving zearalenone per os at the doses of 25 µg/kg b.w. and 50 µg/kg b.w., respectively for 100 d. The concentrations of zearalenone, progesterone, and 17β- oestradiol were analysed in weekly intervals. Zearalenone was noted as early as minute 30 and then during the whole experiment. High concentration of zearalenone (9.34 - 124.33 ng/mL.) was observed in weeks 6-9. The intoxication was accompanied by hormonal disturbances due to progesterone concentration (to 25-30 ng/mL) depending on zearalenone dose and by the increasing in 17β-oestradiol concentration (to 33 pg/ml). Hormonal disturbances of this kind are similar to those noted in different pathological conditions in the genital tract in bitches.
The timing of early reproductive events for the Natal long-fingered bat, Miniopterus natalensis, was examined during its breeding season (August to December) in its maternity roost at the De Hoop Nature Reserve (South Africa). This migrating species is monoestrous with copulation occurring around April in South African populations. Embryonic development is stalled during hibernation by delayed implantation of the blastocyst. As a result, limb bud stage embryos (CS13) are first noted in other South African populations in mid-September. The timing of these reproductive events for the De Hoop population is similar with bats arriving at the maternity roosts in September and embryos at the limb bud stage (CS13) being noted in this month. Pregnancy was not synchronous in the population with bats dissected on the same day exhibiting differences in the stage of development of their young (CS11 to CS21 being the largest range). Maternal features (progesterone concentration, body mass and abdominal distension) were analysed to determine if they provided a reliable estimate of embryonic stage. The progesterone concentrations of pregnant bats showed a similar profile to the Kwa-Zulu Natal and the Eastern Cape populations. Though progesterone concentration did increase as pregnancy progressed, it was not an accurate predictor of embryonic stage. Maternal body mass did not correlate with the stage of embryonic development. However, abdominal distension determined by palpation is an accurate field based predictor of the stages of pregnancy: no abdominal distension, small to medium abdominal distension and large abdominal distension distinguished between non-pregnant bats and those carrying either early (CS11–CS15) or mid-developmental stages (CS16–CS21), respectively.
This study aimed to compare the effectiveness of ovariohysterectomy operations with aglepristone and aglepristone+PGF₂ₐ treatment protocols, preceded by clinical, gynaecological, and ultrasonographical examinations and total blood and hormone analyses in bitches. Furthermore, the influence of initial progesterone (P4) concentrations and uteral diameters on the efficacy of pyometra treatment was determined. Thirty bitches with pyometra were divided into three equal groups: the surgically treated group (OP) and groups treated pharmacologically aglepristone (AL) and with aglepristone+PGF₂ₐ (AP). A dose of 10 mg/kg of aglepristone was administered subcutaneously on days 1, 2, and 7 and if needed on day 14 (groups AL and AP). The bitches of the AP group received additionally 0.25 mg/kg of PGF₂ₐ, dinoprost trometamin, once every 24 h between days 3 to 7. Eight bitches in the OP group (80%), five bitches (50%) in the AL group, and five bitches (50%) in the AP group recovered. The success ratios we established, ignoring the criteria for treatment acceptance, increased to 60% in the AL group and 83.3% in the AP group when the bitches with P4>2 ng/mL and without ovarian cysts were evaluated and the difference between success ratios of the two groups was found to be insignificant. It has been found that carrying out frequently repeated examinations, pharmacological treatments using aglepristone or aglepristone+PGF₂ₐ constitutes a safe alternative to ovariohysterectomy in bitches in the dioestrus phase with P4>2 ng/mL and without ovarian cysts.
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