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1
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Effect of tianeptine and fluoxetine on the levels of met-enkephalin and mRNA encoding proenkephalin in the rat

100%
Dziedzicka-Wasylewska M., Dlaboga D., Pierzchala-Koziec K., Rogoz Z.
Journal of Physiology and Pharmacology
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2002
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tom 53
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nr 1
The purpose of the present study was to evaluate the effects of acute and repeated treatment with two antidepressant drugs (ADs)of opposite pharmacological profile,i.e. tianpetine (TIA,serotonin reuptake enhancer)and fluoxetine (FLU,serotonin reuptake inhibitor)on the levels of Met-Enkephalin,(Met-Enk,a member of opioid peptide family, which has been suggested to play a role in the mechanism of action ADs)as well as on mRNA coding for proenkephalin (mRNA PENK)in various regions of the rat brain, pituitary,adrenal glands and plasma.Male Wistar rats were treated acutely or repeatedly (10 mg/kg p.o.,twice daily for 14 days)with TIA or FLU.Tissue for biochemical experiments was taken 2 h after last dose of appropriate drug.The levels of Met-Enk were estimated by radioimmunoassay,mRNA PENK was measured using in situ hybridization.From the results obtained in the present study it may be concluded that repeated administration of TIA or FLU induced similar changes in the levels of Met- Enk in the rat hippocampus,striatum,hypothalamus and neurointermediate lobe of pituitary.Such an effect is interesting,especially if one takes into account the differences in pharmacological profile between these two antidepressant drugs.It may be suggested that serotonin level might not be crucial for inducing the alterations in the content of Met-Enk.Since we did not observe any changes in the levels of PENK mRNA in the studied rat brain regions after repeated administration of TIA or FLU,it seems that the observed changes in the levels of Met-Enk do not result from effects of these antidepressants on biosynthesis of PENK,but rather from alterations in the peptide release.Another interesting finding of the present study was that in the anterior lobe of pituitary,adrenal glands and plasma,repeated administration of TIA induced alterations in the contents of Met-Enk,while repeated administration of FLU remained without any effect.It is tempting to speculate that such a differentiation between the effects of these two antidepressants might be linked to the well known feature of TIA (but not FLU)which has been shown to reduce both basal and stress-evoked activity of the hypothalamic-pituitary-adrenal (HPA)axis.
2
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The expression of genes coding for opioid precursors, opioids receptors, beta-LH subunit and GnRH receptor in the anterior pituitary of cyclic gilts

84%
Wylot B., Staszkiewicz J., Okrasa S.
Journal of Physiology and Pharmacology
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2008
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tom 59
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nr 4
745-758
In previous studies the effect of endogenous opioid peptides (EOP) on LH secretion was mainly considered at the hypothalamic level, while opioid involvement in the modulation of LH secretion at the pituitary level remains insufficiently elucidated. Therefore, the present study was undertaken to determine the expression of genes encoding opioid precursors – proopiomelanocortin (POMC), proenkephalin (PENK), prodynorphin (PDYN) and opioid receptors – mu, delta, kappa in the porcine anterior pituitary throughout the estrous cycle. Additionally, the mRNA content of ß-LH subunit and GnRH receptor (GnRH-R) was estimated. Pituitaries (5×N = 7) were collected from sows on days 3-5, 8-10, 13-15, 16-17 and 19-20 of the cycle and gene expression was determined using a semi-quantitative RT-PCR assay. The expression of POMC, PDYN, delta and kappa receptor genes was variable across the cycle, whereas the expression of PENK and mu receptor genes remained relatively stable. The POMC mRNA content was the lowest on days 19-20 of the cycle and the PDYN content was reduced on days 8-10. The delta receptor mRNA content was elevated on days 3-5, while the kappa receptor mRNA content was decreasing over the luteal phase. Changes in the expression of genes encoding ß-LH and GnRH-R were also demonstrated. These results indicate variable activity of pituitary opioid systems in cyclic pigs and suggest implication of EOP in the modulation of LH secretion at the pituitary level.
3

Effect of prolonged progesterone treatment on the proenkephalin mRNA gene expression and enkephalins concentration in the sheep brain

67%
Pierzchala-Koziec K., Zubel J., Rzasa J.
Reproductive Biology
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2006
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tom 06
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nr Suppl.2
4
Content available remote

The expression of genes encoding opioid precursors and the influence of opioid receptor agonists on steroidogenesis in porcine adrenocortical cells in vitro

67%
Krazinski B. E., Koziorowski M., Brzuzan P., Okrasa S.
Journal of Physiology and Pharmacology
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2011
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tom 62
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nr 4
5

Regulation of proenkephalin gene expression by transcription factors Fos and CREB: an antisense oligonucleotide approach

67%
Ziolkowska B., Przewlocka B., Bilecki W., Machelska H., Przewlocki R.
Biotechnologia
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1996
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nr 4
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