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Comparison of methods for determining the content and polymerization of proanthocyanidins and catechins

100%

Oszmianski J., Bourzeix M.

Polish Journal of Food and Nutrition Sciences

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1996

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tom 05

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nr 2

The methods with vanillin (V), Folin-Ciocalteau (FC) or dimethyla- minocinnamaldehyde (DC) reagent and spectometric method at 280 nm (A) for determining the content of proanthocyanidins and catechins and their polymerization degree were compared. The (+) catechin, (-)epicatechin and their dimer and trimer standards, and grape wine, wine concentrate and powdered wine were examined. The DC reagent appeared most sensitive in the catechin and proanthocyanidin analysis with the molar absorption values for catechins being 55-fold higher than for the FC reagent In the proanthocyanidin analysis with the DC reagent the use of catechin standard curve is not recommended for the determination of proanthocyanidin content because the concentrations of 0.5 g/L for dimers and 0.3 g/L for trimers were obtained instead of 1 g/L in model solutions. Hie V/DC value approximated most closely the real values of proanthocyanidin polymerization.

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The effect of cis-jasmone, jasmonic acid and methyl jasmonate on accumulation of anthocyanins and proanthocyanidins in seedlings of common buckwheat [Fagipyrum esculentum Moench]

84%

Horbowicz M., Mioduszewska H., Koczkodaj D., Saniewski M.

Acta Societatis Botanicorum Poloniae

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2009

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tom 78

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nr 4

271-277

Effects of various jasmonates (methyl jasmonate, jasmonic acid, cis-jasmone) on anthocyanins and procyanidins content of, as well as on growth of common buckwheat (Fagopyrum esculentum Moench) seedlings were studied. The studied jasmonates were applied as solutions or vapors on four days seedlings, and the seedlings were grown during the next four days in day/night conditions (16/8 h). Afterwards anthocyanins and proanthocyanidins content, as well as elongation of primary roots and hypocotyls were measured. When applied as solutions cis-jasmone (JAS) stimulated the anthocyanins accumulation, but when used as vapors had tendency to decrease its accumulation in buckwheat hypocotyls. Jasmonic acid (JA) solutions slightly stimulated or had no effect on biosynthesis of anthocyanins in buckwheat hypocotyls, but used as vapors caused a decline of anthocyanins in buckwheat hypocotyls. Methyl jasmonate (MJ) clearly inhibited biosynthesis of anthocyanins in hypocotyls of buckwheat seedlings. The studied jasmonates had no influence on anthocyanins level in cotyledons of buckwheat seedlings, except cis-jasmone, which at the lowest solution concentration slightly enhanced biosynthesis of the pigments. Treatment of buckwheat seedlings with solutions of all jasmonates (10-8 M, 10-6 M and 10-4 M) had no influence on the growth of buckwheat hypocotyls. Contrary to that observation vapors of the growth regulators in concentrations 10-4 M, had a strong inhibitory effect on the growth of hypocotyls of buckwheat seedlings. Solutions of JA and MJ, as well as vapors of JA, MJ and JAS strongly inhibited the primary root growth of buckwheat seedlings, while JAS applied as solution had no such influence. MJ and JA caused much higher stimulation of proanthocyanidin biosynthesis in buckwheat hypocotyls than JAS.

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Comparison of proanthocyanidins content in Rhodiola kirilowii and Rhodiola rosea roots - application of UPLC-MS/MS method

84%

Gryszczynska A., Krajewska-Patan A., Buchwald W., Czerny B., Mielcarek S., Rudzinska K., Mroziewicz P. M.

Herba Polonica

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2012

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tom 58

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nr 3

The purpose of presented study was the comparison of flavan-3-ol content in the roots of Rhodiola kirilowii and Rhodiola rosea with use of UPLC-MS/MS method. Two kinds of extract were prepared: aqueous extract and 50% v/v ethanol extract. The worked out UPLC MS/MS method allowed to determine the content of five flavan-3-ols: (+)-catechin, (-)-epicatechin, (-)-epigallocatechin, (-)-epicatechin gallate (ECG) and (-)-epigallocatechin gallate (EGCG). The obtained results shown that the content of measured catechins was higher in R. kirilowii roots than in R. rosea. Both Rhodiola roots contain EGCG as a main proanthocyanidin compound – the content in R. kirilowii roots is about 0.14%. Our results indicate that application of ultra performance liquid chromatograph connected to a tandem mass spectrometer (UPLC MS/MS method) allows to determine the proanthocyanidins content in tested samples with satisfactory precision and can be used in the Rhodiola sp. phytochemical investigations.

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Proanthocyanidins in Rhodiola kirilowii and Rhodiola rosea callus tissues and transformed roots - determination with UPLC-MS/MS method

84%

Gryszczynska A., Krajewska-Patan A., Dreger M., Buchwald W., Pietrosiuk A., Zych M., Karasiewicz M., Bogacz A., Kujawski R., Furmanowa M., Czerny B., Mielcarek S., Gazda P., Mrozikiewicz P. M.

Herba Polonica

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2012

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tom 58

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nr 4

Several species of Rhodiola genus (Crassulaceae family) like Rhodiola kirilowii and Rhodiola rosea are used in official or traditional medicine. The aim of this study was to determine qualitative and quantitative content of proanthocyanidins using ultra performance liquid chromatograph connected to a tandem mass spectrometer (UPLC MS/MS method) in the callus tissues and in the transformed roots (infected by Agrobacterium rhizogenes LBA 9402 strain) of R. kirilowii and R. rosea. This validated assay allows to determine the content of five flavan-3-ols: (+)-catechin, (-)-epicatechin, (-)-epigallocatechin, (-)-epicatechin gallate (ECG), (-)-epigallocatechin gallate (EGCG). Our results concerning the material from in vitro cultivation of R. kirilowii and R. rosea indicate that R. rosea callus can be a better source of catechin when compared with other tested materials, especially when the content of (-)-gallate epigallocatechin is taken under consideration (3.429 mg/100 g of dry powdered material). The application of UPLC MS/MS method allowed to determine the content of proanthocyanidins in tested samples with satisfactory precision and can be used in the phytochemical investigations of Rhodiola sp. in vitro cultivated tissues.

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Influence of Viburnum opulus proanthocyanidins on stress-induced gastrointestinal mucosal damage

84%

Zayachkivska O. S., Gzhegotsky M. R., Terletska O. I., Lutsyk D. A., Yaschenko A. M., Dzhura O. R.

Journal of Physiology and Pharmacology. Supplement

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2006

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tom 57

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nr 5

155-167

Recent studies demonstrated that the proanthocyanidins (PA), the polymers of flavan-3-ols, naturally occurring plant metabolites widely available in fruits, vegetables, nuts, seeds, flowers and bark, have anti-inflammatory, anticarcinogenic, anti-allergic, antioxidant and vasodilatory actions. We hypothesized that Viburnum opulus PA (VOPA, Caprifoliaceae), due to activation of multifactorial gastrointestinal mucosal defense mechanisms, exert gastroduodenoprotective effects. The aim of the study was: 1) to investigate VOPA effects on gastroduodenal mucosal integrity and pattern of carbohydrate binding proteins and nitric oxide (NO) content in intact mucosa and that exposed to non-topical ulcerogens (stress) in rats without and with capsaicin (125 mg/kg, sc) denervation; and 2), to assess the role of activity of antioxidizing enzymes superoxide dismutase (SOD), catalase (CAT), gluthatione peroxidase (GPx) in VOPA-iduced gastroduodenoprotection against water immersion and restraint stress (WRS) in rats. VOPA was administered orally in dose of 25, 50 or 75 mg/kg body weight. Gastroduodenal mucosal damage detected by routine light microscopic investigation and lectin histochemistry set, purified from plant and animal sources of Carpatian region. NO content, pro-and antioxidant system were determinated by routine laboratory methods. Pretreatment with VOPA afforded gastroduodenoprotection and was accompanied by an increase in NO expression, both changes being reversed by sensory denervation, as well as by the rise of SOD, CAT activity and fall in MDA content. Our study shows that VOPA exerts a potent gastroduodenoprotective activity via an increase in endogenous NO generation, suppression of lipid peroxidation and mobilization of antioxidant activity and changes in glycoconjugate content of the gastroduodenal mucosa of rat.

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Analiza HPLC antocyjanidyn po hydrolizie proantycyjanidyn gryki i kaszy gryczanej

59%

Amarowicz R.

Bromatologia i Chemia Toksykologiczna

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2006

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tom 39

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nr 2

189-195

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Soki owocowe o wysokiej aktywnosci biologicznej

43%

Oszmianski J.

Przemysł Fermentacyjny i Owocowo-Warzywny

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2007

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tom 51

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nr 04

12-14

Epidemiological studies have indicated that fruits, vegetables and their products as juices consumption is associated with reduced risk of chronic diseases. Juices are besides fruits and vegetable suitable food products in term of ingestion of health protective phytochemicals.. They are good source of biological active compounds especially of polyphenols with anioxidants activity, free radical scavengers and metal chelators; they exhibit various physiological activities including anti-inflamatory, antiallergic, anticarcinogenic and antiarthritic activities. Berry fruit juices are one of the richest source of natural antioxidants, must be drinking in lager quantities, they are very important for the maintenance of health and prevention of disease. For increasing bioactive compounds in apple products it is necessary to improvement of clear apple juice processing and production or cloudy apple juice without clarification.