Dioxins and PCBs are ubiquitous, persistent organic contaminants of food, mainly of animal origin. Chronic toxicity of trace levels of these compounds, manifested by disruption of the human endocrine system homeostasis, is an important public health problem. EU food legislation for over 10 years sets maximum levels for the most toxic representatives of these compounds in food. The purpose of this paper is to present the newest legislation on maximum residue levels for polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and polychlorinated biphenyls (PCBs), which entered into force in Member States in 2012, and to explain the main assumptions supporting its setting.