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The aim of the present study was to evaluate the degree of microbial air contamination in three laboratory rooms designed for raising broiler chickens under identical conditions, in the summer and winter. It was found that in identical poultry houses and under identical management conditions, certain differences can be observed with regard to temperature and humidity parameters and the degree of microbial air contamination, both in the summer and winter. The concentrations of aerobic mesophilic bacteria and fungi were higher in the winter than in the summer in all rooms. Various levels of microbial air contamination had no effect on broiler production results.
Since intensive poultry production is accompanied by as high as possible densities of birds within buildings, this exposes poultry house workers to elevated concentrations of bioaerosol that is mainly emitted by birds. Exposure to dust containing pathogenic microbial and parasitic agents may cause asthma, asthma-like syndrome, mucous membrane irritation, chronic bronchitis, and allergic alveolitis organic dust toxic syndrome, as well as chronic obstructive pulmonary diseases. Since the microbial air pollution data base of poultry houses is insufficient at present, and poultry production is increasingly widespread, it is important to collect, compare and update the available data.
The aim of the presented study was to assess the exposure of poultry workers to airborne microorganisms, endotoxins and β-glucans during different stages of the chicken production cycle in 3 commercially-operated poultry houses. Personal and stationary sampling was carried out to assess exposure to both viable and total microbial aerosols. The stationary measurements of PM10 were performed to establish the level of endotoxins and β-glucans. The concentrations of bacterial and fungal aerosols ranged from 2.5×102 CFU/m3 – 2.9×106 CFU/m3, and from 1.8×102 CFU/m3 – 1.8×105 CFU/m3, respectively. The number of culturable microorganisms was significantly lower than their total counts, constituting from 0.0004% – 6.4% of the total microbial flora. The level of PM10 in poultry facilities did not exceed 4.5 mg/m3. After the flock entered the clean house, the level of endotoxins and β-glucans increased from below detection limit to 8,364 ng/m3 and from 0.8 ng/m3 to 6,886 ng/m3, respectively. The presented study shows that professional activities in poultry farms are associated with constant exposure to bioaerosol, which may pose a health hazard to workers. It was found that workers’ exposure to airborne microorganisms increased with consecutive stages of the chicken production cycle.
Investigations on airborne fungi in a poultry house, a swinery, a feed preparing and storing house, a grain mill, a wooden panel producing factory, and organic waste recycling facilities have been carried out in Lithuania. Low concentrations of fungal spores were detected in the wooden panel producing factory, the swinery, the feed preparing and storing house, and the poultry house; moderate concentrations were found in the organic waste recycling facilities; high concentrations were revealed at the grain mill. Species of Aspergillus oryzae, A. nidulans, P. expansum, Penicillium olivinoviride, P. claviforme and Botryotrichum longibrachiatum prevailed in the poultry farm; Geotrichum candidum, Cladosporium cladosporioides, C. herbarum, Penicillium viridicatum and P. fellutanum dominated in the swinery. Fungi of Penicillium viridicatum, P. expansum, Staphylotrichum coccosporum and Aspergillus oryzae prevailed in the feed preparing and storing house at the swinery. Cladosporium cladosporioides, C. herbarum, Penicillium viridicatum and Geotrichum candidum prevailed in the grain mill. Fungi ascribed as Paecilomyces puntonii, Rhizopus nodosus and R. stolonifer dominated in the wooden panel producing factory. Species of Aspergillus raperi, P. paxilli, P. oxalicum, and Cladosporium herbarum prevailed at the organic waste recycling facilities. According to published data, the majority of the identified fungal species are characterized as allergenic and an exposure to their spores may provoke adverse health effects (such as allergic rhinitis, bronchial asthma or extrinsic allergic alveolitis) in susceptible individuals.
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