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1

Potassium balance at some farms in Poland

100%
Labetowicz J., Majewski E., Rutkowska B., Szulc W., Kalwara A.
Journal of Elementology
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2005
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tom 10
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nr 4 Suppl.1
58
2

Effect of different K-supply on sugar beet production and soil fertility in a long-term fertilizer experiment

72%
Von B. L. C., Orlovius K.
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
|
2002
|
tom 222
In a long-term field experiment with a duration of 19 years the impact of different rates of potassium (0–100–200–300 kg K2O/ha/year) with and without farmyard manure (FYM) (30 t/ha every 3 years before sugar beet) on yields, crop quality, K-content in the soil and the economic effects in a sugar beet — wheat — barley crop-rotation was studied. K-rates below crops K-uptake led to a distinct decline in soil fertility. Sugar beet as well as wheat showed an average yield decrease of 35% and 12%, resp. during the whole trial period. FYM increased the yield level especially of sugar beet, but did not affect the optimum K-rate. With negative K-balances and a falling K-content in the soil, sugar yields decreased over the whole trial period combined with a lower sugar content in the roots. For that reason the efficiency of K-fertilization increased with the duration of the experiment. At the described site an extractable K-content of about 250 ppm K2O was optimum. In order to maintain this soil-K-value a K-application rate exceed the K-removal by 45 kg K2O/ha/year was necessary.
3

Spectrophotometric method for the determination of renal ouabain-sensitive Hplus, Kplus-ATPase activity

72%
Beltowski J., Wojcicka G.
Acta Biochimica Polonica
|
2002
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tom 49
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nr 2
The aim of this work was to develop a method for renal H+,K+-ATPase measurement based on the previously used Na+ ,K+ -ATPase assay (Bełtowski et al.: J Physiol Pharmacol.; 1998, 49: 625-37). ATPase activity was assessed by measuring the amount of inorganic phosphate liberated from ATP by isolated microsomal fraction. Both ouabain-sensitive and ouabain-resistant K+ -stimulated and Na+ -independent ATPase activity was detected in the renal cortex and medulla. These activities were blocked by 0.2 mM imidazolpyridine derivative, Sch 28080. The method for ouabain- sensitive H+ ,K -ATPase assay is characterized by good reproducibility, linearity and recovery. In contrast, the assay for ouabain-resistant H ,K -ATPase was unsatisfac­tory, probably due to low activity of this enzyme. Ouabain-sensitive H+ ,K+ -ATPase was stimulated by K + with Km of 0.26 ± 0.04 mM and 0.69 ±0.11 mM in cortex and me­dulla, respectively, and was inhibited by ouabain (Ki of 2.9 ± 0.3 uM in the renal cortex and 1.9 ± 0.4 uM in the renal medulla) and by Sch 28080 (Ki of 1.8 ± 0.5 uM and 2.5 ± 0.9 uM in cortex and medulla, respectively). We found that ouabain-sensitive H+ ,K+ -ATPase accounted for about 12% of total ouabain-sensitive activity in the Na+ ,K+-ATPase assay. Therefore, we suggest to use Sch 28080 during Na+,K+-ATPase measurement to block H+ ,K+ -ATPase and improve the assay specificity. Leptin ad­ministered intraperitoneally (1 mg/kg) decreased renal medullary Na+ ,K+ -ATPase ac­tivity by 32.1% at 1 h after injection but had no effect on H+ ,K+ -ATPase activity sug­gesting that the two renal ouabain-sensitive ATPases are separately regulated.
4
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Enhanced expression of mineralocorticoid receptors in the heart after the myocardial infarct in rats

58%
Milik E., Szczepanska-Sadowska E., Maslinski W., Cudnoch-Jedrzejewska A.
Journal of Physiology and Pharmacology
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2007
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tom 58
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nr 4
Increasing evidence suggests that enhanced stimulation of the heart and kidney by mineralocorticoids plays significant role in development of the post-infarct cardiac failure. Because increased synthesis of mineralocorticoid receptors (MR) is one of the putative factors determining pathogenic effects of mineralocorticoids we decided to determine whether the myocardial infarct results in an enhanced expression of MR mRNA and MR protein. To this end male Sprague-Dawley rats were subjected either to ligation of the left coronary artery or to sham surgery. After four weeks expressions of MR mRNA and MR protein were evaluated in both groups of rats in the left (LV) and right (RV) ventricle walls, and in the renal cortex and renal medulla by means of semiquantitative PCR and Western blotting methods. Coronary ligation resulted in the myocardial infarction encompassing 30.2% ± 1.9% (range 23-40%) of the left ventricle wall. In the infarcted rats expression of MR mRNA was significantly greater than in the sham-operated rats, both in the LV (P<0.02) and in the RV (P<0.005). In the left but not in the right ventricle increased MR mRNA expression was associated with significant increase in expression of MR protein (P<0.001). In the renal cortex and renal medulla MR mRNA and MR protein expression in the infarcted and the sham-operated rats did not differ. The study reveals that during the post-infarct state expression of MR mRNA is elevated in both cardiac ventricles while expression of MR mRNA protein is increased only in the left ventricle. The results suggest that the enhanced expression of mineralocorticoid receptors may contribute to enhanced effects of mineralocorticoids in the heart during the post-infarct state.
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