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In 2007–2009, a pot experiment was carried out in a greenhouse at the University of Warmia and Mazury in Olsztyn on cv. Promyk pot marigold (Calendula officinalis L.). The aim of the study was to assess the impact of nitrogen and magnesium in plant growth and development and yield of marigold. The experiment included two factors: nitrogen fertilization (g.pot-1): A–0 (control), B–0.3, C–0.6 (0.6+0), D–0.9 (0.6+0.3), E–1.2 (0.6+0.6) and magnesium fertilization (g.pot-1): a–0 (control), b–0.5. The experimental part of the research was designed according to the independent series method and set up in 4 replicates, 4 pots in one replicate, in modified Kick-Brauckmann pots. The experiment demonstrated a significant effect of nitrogen fertilization on the plants hight, number of pot marigold inflorescences, fresh inflorescences weight, fresh and airdry ligulate weight. Magnesium fertilization had a positive effect on all the analyzed traits. No statistical correlation was proven between the nitrogen and magnesium fertilization.
Calendula officinalis L. is one of the most valuable medicinal plants of Asteraceae family and is widely used in the pharmaceutical, health, and food industries. To evaluate the effect of different treatments of super absorbent and zinc sulfate fertilizer on the yield and yield components of Calendula officinalis L., this experiment was conducted at the Islamic Azad University of Khoy. This was a factorial experiment using as the first factor super absorbent polymers at four levels to the soil (0, 4, 6, 8 g/kg) and as the second factor, 4 zinc sulfate concentrations of 0, 25, 50, and 70 mg per pot (based on a randomized complete block design with three replications). The results showed that super absorbent polymers had significant effect on all yield traits, but Zn sulfate fertilizer had only a significant effect on flower diameter, number of stems, total biomass with seeds, total biomass yield, seed yield, and harvest index. The interaction of the super absorbent polymers and zinc sulfate fertilizer increased the plant height, total biomass with seeds and flowers, seed weight, and flower and seed harvest index, whereas the diameter of flowers, number of stems, dry flower yield, flower number per m2, and seed yield were not affected. Super absorbent polymers with zinc sulfate fertilizer had the greatest effect on most yield traits and had a positive effect on increasing the yield components of Calendula officinalis L., which improved the grain yield. The use of super absorbent polymers can improve the water holding capacity of the soil, which reduces the need for water and improves the yield in arid and semi-arid areas.
The aim of this study was to examine the effects of management practices and biofertilization on microbial activity in rhizosphere and yield of medicinal and aromatic plants. Field experiment was performed using four plant species: peppermint (Mentha × piperita L.), pot marigold (Calendula officinalis L.), sweet basil (Ocimum basilicum L.), and dill (Anethum graveolens L.). Treatments were arranged in a split-plot layout in four replicates using basic plots under conventional and organic management, and subplots with and without biofertilizer (Azotobacter chroococcum). Organic management positively affected the microbial number and activity. Biofertilization increased the total microbial number (13–21%), number of ammonifiers (13–60%), nitrogen-fixing bacteria (7–36%), actinomycetes (36–50%), fungi (60–100%), cellulolytic microorganisms (57–217%), dehydrogenase (28–52%) and ß-glucosidase activity (15–39%). The effects of management practices and biofertilization were highly significant for the yield of examined plants. The yields were higher on inoculated treatments both in conventional (5–26%) and organic (7–15%) growing system.
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