Contamination with bioaerosols most affects the livestock production, and especially the poultry. Prolonged or repeated exposure to high concentrations of airborne fungal spores is considered a major risk factor for human health and contributes to the deterioration of lung functions, and particularly allergic diseases. The aim of the study was to determine the number and quality of moulds and yeasts in the air of various objects for poultry post-slaughter waste processing. The study allowed to detect the presence of moulds and yeasts in the air of all sampling points. There were: Aspergillus sp., Penicillium sp., Scopulariopsis sp., Trichoderma sp., Acremonium sp., Cephalosporium sp., Alternaria sp., Pithomyces sp., Eurotium sp., and: Rhodotorula sp., Candida sp., Yarrovia sp. and Saccharomyces sp. Statistical analysis confirmed the presence of highly significant influence of the sampling point, date, and interaction of these factors on the fungi population.