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Flow cytometric analysis of leukocytes in porcine mammary secretions

100%

Pomorska-Mol M., Markowska-Daniel I., Bednarek D.

Bulletin of the Veterinary Institute in Puławy

2010

tom 54

nr 2

188-192

Nine sows were monitored for leukocyte subsets in mammary secretions during lactation. Using flow cytometry, percentages of monocytes/macrophages (CD45⁺CD14⁺), T cells (CD3⁺), and their subpopulations (CD4⁺, CD8⁺ and double positive (DP) CD4⁺CD8⁺), and B cells (CD21⁺) in colostrum and milk were determined. The mean percentage of CD3⁺cells was relatively stable throughout lactation. About 40% of colostral lymphocytes were identified as CD3⁺, while in milk they comprised from 24% to 39%. The percentage of both CD8⁺ and CD4⁺ cells were relatively stable during lactation, beside significant decrease at the last day of lactation. Double positive cells comprised a small percentage of cells and did not exceed 7.5%. The frequency of DP lymphocytes increased significantly 6 h after parturition, and then remained relatively stable until 20th d of lactation, followed by a significant decrease at day 28 postpartum. The CD8⁺ cells prevailed over CD4⁺ cells throughout whole lactation. The CD4/CD8 ratio was similar during lactation period and ranged from 0.50 to 0.53 at the beginning and the end of lactation, respectively. Lymphocytes B represented about 20% of all lymphocytes of mammary secretions. CD45⁺CD14⁺ cells ranged between 5.01 and 10.14% of all leukocytes.

Shifts in immunoglobulins levels in the porcine mammary secretions during whole lactation period

80%

Markowska-Daniel I., Pomorska-Mol M.

Bulletin of the Veterinary Institute in Puławy

2010

tom 54

nr 3

The absolute and relative levels of immunoglobulins (Ig) G, M, and A in porcine mammary secretions throughout lactation period were estimated. Concentrations of each immunoglobulin were determined by ELISA kits. The results of the study confirmed that pigs, like other mammals, have colostral and milk phases of lactation, distinguished by the concentrations of IgG and IgA. The amount of IgG showed a dramatic 27-fold decline from the beginning to day 6 of lactation. The IgM concentration decreased over threefold at the same time. Concentration of IgA at the day 6 of lactation was almost 4-fold lower than that at the beginning of lactation. The relative contribution of IgG and IgA to the total Ig content becomes reversed during lactation. Summarising, in pigs, transformation of colostrum to milk was characterised by dramatic decrease in total Ig content, connected with the change of predominant immunoglobulin. The predominant immunoglobulin in colostrum was IgG, which plays an important role in systemic immunity, while in milk a major Ig was IgA, responsible for the protection at mucosal surface level. As concentrations of Ig in colostrum are very variable, an improved understanding of factors influencing colostrum Ig concentration is now desirable.

Ograniczanie wyników

2 Bulletin of the Veterinary Institute in Puławy

2 Markowska-Daniel I.

2 Pomorska-Mol M.

1 Bednarek D.

2 2010

Wyniki wyszukiwania

Flow cytometric analysis of leukocytes in porcine mammary secretions

Shifts in immunoglobulins levels in the porcine mammary secretions during whole lactation period