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Two hundred and fifteen rabbit sera have been assessed by the haemagglutination inhibition test for the presence of antibodies against lagomorphs disease virus. Sera were inactivated at 57—58°C for 30 minutes and the haemagglutination inhibition test was performed with human erythrocytes of group 0 (Rh +). The erythrocyte suspension was prepared in Sörensen’s buffer, pH 5.8, containing 0.1 per cent of human serum albumin. The presence of the antibodies was revealed in 31.6 per cent sera; their titers ranged from 10 to 6400 units.
A study on the seroprevalence of VHDV antibodies in rabbits in 5 different regions of Central and South-Eastern parts of Poland was undertaken. A total of 2180 samples of sera collected from animals small private farms were tested. Out of 630 examined samples of sera from large farms, 270 were taken from rabbits older than 2-months born from seropositive dams. The VHDV antibodies were recorded in 138 (6.3%) samples of sera with the level of various provinces of Poland from 3 to 11.2 %. We found out that the presence of the seropositive animals was due to VHDV infection as well as the prophylactic vaccination employed during the last years.
RHD virus (RHDV), which causes rabbit hemorrhagic disease, is the object of phylogenetic studies. The results of these studies provide valuable information on this pathogen. The purpose of present review is to summarize current knowledge about the phylogenetic position of this virus. The paper discusses the results of previous phylogenetic studies of RHDV, including factors affecting the shape of the phylogeny of this species and the diversity of RHDV strains in different parts of the world. It also presents the results of phylogenetic analysis of RHDV enriched with non-pathogenic and low pathogenic strains of rabbit lagoviruses, as well as phylogenetic analyses performed using modern Bayesian methods. The paper also highlights the unclear situation of Lagovirus taxonomy and shows selected data on the evolution of RHDV, gathered using phylogenetic methods. Finally, the possibility of using rabbit lagoviruses as a model for the study of the evolution of mammalian ssRNA(+) viruses has been shown.
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