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Effect of DNA quality on randomly amplified polymorphic DNA-based identification of strawberry [Fragaria x ananassa] genotypes

100%

Korbin M., Kuras A., Golis A., Zurawicz E.

Journal of Fruit and Ornamental Plant Research

2000

tom 08

nr 3-4

Trichinella britovi and T.spiralis in red foxes [Vulpes vylpes] in Poland

80%

Cabaj W., Pozio E., Moskwa B., Malczewski A.

Acta Parasitologica

2000

tom 45

nr 4

The aim of the present survey was to determine the distribution of Trichinella species in red foxes in Poland. Muscles have been collected from 1282 red foxes (Vulpes vulpes) killed by hunters in different regions of Poland between 1995 and 1999. Trichinella larvae have been collected after artificial digestion of muscles and preserved in absolute ethyl alcohol before molecular identification. Random amplified polymorphic DNA analysis and two other polymerase chain reaction (PCR)-based analyses have been used to identify larvae at the species level. The overall prevalence of infection was found to be 5.7% (73/1282 examined foxes), but larvae from only 44 foxes could be recovered for species identification. From these, thirty-two foxes (72.7%) harboured T. britovi, five (11.4%) had T. spiralis, four (9.1%) showed a mixed infection with the two species. The DNA of larvae from three (6.8%) animals did not showed reproducible results. This study shows T. britovi as the most important etiological agent of sylvatic trichinellosis in almost the whole territory of Poland and the role of red fox in the maintenance of this parasite in nature.

A phylogenetic analysis of trematodes of the genus Echinoparyphium and related genera based on sequencing of Internal Transcribed Spacer region of rDNA

80%

Grabda-Kazubska B., Borsuk P., Laskowski Z., Mone H.

Acta Parasitologica

1998

tom 43

nr 3

The sequencing of ITS1 fragments of the rDNA were assayed in order to discriminate species and to establish relationships between representatives of the genera: Echinoparyphium (E. elegans, E. recurvatum, E. pseudorecurvatum), Pseudechinoparyphium (P. echinatum), Neoacanthoparyphium (N. echinatoides) and Hypoderaeum (H. conoideum). The assay has shown that the most closely related are three species of Echinoparyphium while P. echinatum and N. echinatoides were more distant, especially the latter. H. conoideum appeared to be close to Echinoparyphium. The analysis of sequences of ITS1 region of rDNA and RAPD profiles corroborated with earlier conclusions based on chaetotaxy of cercariae and the excretory system patterns.

Ograniczanie wyników

2 Acta Parasitologica

1 Journal of Fruit and Ornamental Plant Research

1 Borsuk P.

1 Cabaj W.

1 Golis A.

1 Grabda-Kazubska B.

1 Korbin M.

1 Kuras A.

1 Laskowski Z.

1 Malczewski A.

1 Mone H.

1 Moskwa B.

1 Pozio E.

1 Zurawicz E.

2 2000

1 1998

Wyniki wyszukiwania

Effect of DNA quality on randomly amplified polymorphic DNA-based identification of strawberry [Fragaria x ananassa] genotypes

Trichinella britovi and T.spiralis in red foxes [Vulpes vylpes] in Poland

A phylogenetic analysis of trematodes of the genus Echinoparyphium and related genera based on sequencing of Internal Transcribed Spacer region of rDNA