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Accumulation of poly(A) RNA in the Cajal bodies and nucleus

100%

Rudzka M., Kolowerzo A., Smolinski D.

Acta Biologica Cracoviensia. Series Botanica. Supplement

2014

tom 56

nr 1

Differential stability of mitochondrial mRNA in HeLa cells

86%

Piechota J., Tomecki R., Gewartowski K., Szczesny R., Dmochowska A., Kudla M., Dybczynska L., Stepien P. P., Bartnik E.

Acta Biochimica Polonica

2006

tom 53

nr 1

The physiological significance and metabolism of oligoadenylated and polyadenylated human mitochondrial mRNAs are not known to date. After study of eight mitochondrial transcripts (ND1, ND2, ND3, ND5, CO1, CO2, ATP6/8 and Cyt. b) we found a direct correlation between the half-lives of mitochondrial mRNAs and their steady-state levels. Investigation of the mt-mRNA decay after thiamphenicol treatment indicated that three transcripts (ND2, ND3 and Cyt. b) are significantly stabilized after inhibition of mitochondrial translation. Careful analysis one of them, ND3, showed that inaccurate processing of the H-strand RNA precursor may occasionally occur between the ND3 and tRNAArg locus leading to synthesis of ND3 mRNAs lacking the STOP codon. However, analysis of the oligo(A) fraction observed in case of the ND3 indicates that partially polyadenylated mRNAs are linked rather to the transcription process than to the translation-dependent deadenylation. Analysis of ND3 mRNA turnover in cells with siRNA-mediated knock-down of the mitochondrial poly(A) polymerase shows that strongly decreased polyadenylation does not markedly affect the decay of this transcript. We present a model where oligoadenylated mitochondrial transcripts are precursors of molecules containing full length poly(A) tails.

Polyadenylation and decay of 26S rRNA as part of Nicotiana tabacum response to cadmium

72%

Lewandowska M., Borcz B., Kaminska J., Wawrzynski A., Sirko A.

Acta Biochimica Polonica

2007

tom 54

nr 4

747-755

In contrast to mRNAs, ribosomal RNAs are generally not considered to be polyadenylated. Only a few recent reports describe non-abundant polyadenylated rRNA-related transcripts that have been detected and characterized in yeast and in human cells. Here we depict the phenomenon of 26S rRNA polyadenylation and degradation that was observed in shoots of Nicotiana tabaccum plants grown in the presence of cadmium. Fragments corresponding to 26S rRNA were identified using suppression subtractive hybridization during screening for genes induced in tobacco plants upon a three-week exposure to 15 μM cadmium chloride. Extracts prepared from the above-ground tissues of cadmium-treated tobacco plants were supposed to contain exclusively polyadenylated mRNAs. Surprisingly, numerous polyadenylated fragments matching parts of 26S rRNA were identified and their presence was confirmed by Northern blot and cDNA amplification techniques. To our knowledge this is the first report on rRNA polyadenylation in plants

Ograniczanie wyników

2 Acta Biochimica Polonica

1 Acta Biologica Cracoviensia. Series Botanica. Supplement

1 Bartnik E.

1 Borcz B.

1 Dmochowska A.

1 Dybczynska L.

1 Gewartowski K.

1 Kaminska J.

1 Kolowerzo A.

1 Kudla M.

1 Lewandowska M.

1 Piechota J.

1 Rudzka M.

1 Sirko A.

1 Smolinski D.

1 Stepien P. P.

1 Szczesny R.

1 Tomecki R.

1 Wawrzynski A.

1 2014

1 2007

1 2006

Wyniki wyszukiwania

Accumulation of poly(A) RNA in the Cajal bodies and nucleus

Differential stability of mitochondrial mRNA in HeLa cells

Polyadenylation and decay of 26S rRNA as part of Nicotiana tabacum response to cadmium