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Pollen morphology and two-dimensional patterns of pollen coat and protoplast proteins in Aegilops kotschyi x Secale cereale amphiploids

100%

Kalinowski A., Klimko M., Wojciechowska B.

Acta Biologica Cracoviensia. Series Botanica

2005

tom 47

nr 2

Amphiploid pollen of Aegilops kotschyi x Secale cereale was compared to the parental form by SEM, and comparatively measured using light microscopy. Pollen grains of amphiploids were larger and more variable in total and pore diameter than the parents. Amphiploid pollen was prolate, subprolate and prolate-spheroidal in shape. The exine of Ae. kotschyi AK-2 and AK-3 had a delicate verrucate surface, whereas Secale cereale S14 exine had a verrucate surface. Amphiploid pollen grain surfaces were more or less similar to those of the parents: delicate verrucate, verrucate and well verrucate. The sculpture of parental and amphiploid pollen grains showed conspicuous granulation. All amphiploids produced pollen with one pore with an operculum, surrounded by a well-defined annulus. Sporophytically produced peptides from the pollen coat and gametophytically produced peptides from the protoplast were analyzed separately by two-dimensional gel electrophoresis. Two accessions ofAe. kotschyi (AK-2, AK-3) showed differences in the 2-D patterns of peptides from both the pollen coat and the protoplast. The majority of pollen coat and protoplast peptides of the parents were detected in the amphiploids, but a number of parental peptides were absent. All the amphiploids possessed peptides in their pollen coat and protoplast not detected in the corresponding pollen fractions of the parents. No relation between colchicine and in vitro amphiploid production and its 2-D patterns was observed. The results of pollen morphology and pollen protein analysis are convergent.

Some approaches to the experimental manipulation of reproductive cells and protoplasts in flowering plants

61%

Zhou C., Yang H. Y.

Acta Biologica Cracoviensia. Series Botanica

2000

tom 42

nr 1

One recent advance in plant embryology is the experimental manipulation of various reproductive cells and their protoplasts under in vitro conditions. These experimental means may be helpful for understanding the developmental biology of sexual plant reproduction, on one hand, and developing novel methods in biotechnology, on the other. This article reviews a series of our works in this field. The article includes manipulation of pollen protoplasts, de-exined pollen, male gametoplasts and female gametoplasts. Each section starts with isolation of the cells/protoplasts and is followed by further manipulations such as culture, fusion, gene transfer, and also some cell biology studies based on and related to these manipulations.

Ograniczanie wyników

2 Acta Biologica Cracoviensia. Series Botanica

1 Kalinowski A.

1 Klimko M.

1 Wojciechowska B.

1 Yang H. Y.

1 Zhou C.

1 2005

1 2000

Wyniki wyszukiwania

Pollen morphology and two-dimensional patterns of pollen coat and protoplast proteins in Aegilops kotschyi x Secale cereale amphiploids

Some approaches to the experimental manipulation of reproductive cells and protoplasts in flowering plants