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Differences in growth and transcriptomic profile of bovine mammary epithelial monolayer and three-dimensional cell cultures

100%
Kozlowski M., Gajewska M., Majewska A., Jank M., Motyl T.
Journal of Physiology and Pharmacology. Supplement
|
2009
|
tom 60
|
nr 1
5-14
Mammary epithelial cells (MECs) are characterized by specific spatial architecture with several distinguishing features such as: polarized morphology, specialized cell-cell contacts, and attachment to an underlying basement membrane. Three dimensional (3D) basement membrane cultures provide a unique opportunity to model the architecture of epithelium in vitro. The aim of this study was to characterize the growth of bovine mammary epithelial cell line BME-UV1 in 3D culture on MatrigelTM and identification of differently expressed genes in bovine MECs forming polarized structures in comparison to conventional monolayer (2D) cell culture. We demonstrate that BME-UV1 cells grown on MatrigelTM form polarized acinar structures during 16 days of culture. A microarray study has proven that the difference in spatial architecture between MECs cultured in monolayer and 3D system is reflected by differences in transcriptomic profile. Microarray data analysis showed 40 differentially expressed genes with statistical significance (p<0.05) and characterized biological functions. Identified genes comprised of cytoskeletal proteins, extracellular matrix components, kinases such as: Rac serine/threonine kinase, SRPK, protooncogene tyrosine-protein kinase ABL1, uridine cytidine kinase and proteins with nucleic acid binding / transcription factor activity. Products of those genes are involved in processes which are known to participate in regulating mammary gland polarization and function.
2

Obosnovanie skhemy vysokochastotnogo diehl'kometrichnogo izmeritelja parametrov kachestva moloka

86%
Yakimec V., Bubnjak T., Drobot I.
Motrol. Motoryzacja i Energetyka Rolnictwa
|
2014
|
tom 16
|
nr 4
3

Verdet constant polarimetric measurement in aqueous solutions of salts

86%
Heldt J., Szczepanski J., Kowalewska G., Zielinski A.
Oceanologia
|
1985
|
tom 20
4

On the possibility of application of a polarimetric method for determination of salinity in sea-water

86%
Zielinski A., Kowalewska G., Heldt J., Szczepanski J.
Oceanologia
|
1986
|
tom 23
5

Changes in ion concentration in the air during the breathing process of a human being

86%
Suchanowski A., Wiszniewski A.
Polish Journal of Environmental Studies
|
1999
|
tom 08
|
nr 4
This work discusses the influence of the human being on concebtrations and mobility of aeroions in the atmosphere. The performed investigations show that the concentration of anions decreases, in the vicinity of the human, on about 26 - 44%. This is the result of their recombination with charges existing in the human body. In the process of breathing there are no crucial changes to the concentration of ions of both polarisation, while there is a conciderable increase, by several hundred percent, in the number of large ions.
6

The changing context of racial segregation: an examination in metropolitan Detroit

86%
Sinclair R.
Geographia Polonica
|
1997
|
tom 69
7

Międzynarodowa Komisja ICUMSA ogłasza zmiany skali cukrowniczej i metody polaryzacji

72%
Player M. R.
Gazeta Cukrownicza
|
1988
|
tom 96
|
nr 11-12
8

Mathematical spectral model of solar irradiance reflectance and transmittance by a wind-ruffled sea surface. Part 1. The physical problem and mathematical apparatus

72%
Wozniak S. B.
Oceanologia
|
1996
|
tom 38
|
nr 4
9

Mozliwosci zastosowania pradu elektrycznego w procesie peklowania miesa

58%
Zywica R., Cierach M.
Acta Academiae Agriculturae ac Technicae Olstenensis. Technologia Alimentorum
|
1995
|
tom 28
49-54
10

Effects of methyl parathion on model membrane fluidity: Polarization of diphenylhexatriene and intramolecular excimerization of a pyrene derivative

58%
Blasiak J.
Current Topics in Biophysics
|
1993
|
tom 17
|
nr 1-2
11

The light scattering matrix of Chlorella vulgaris cells and its variability due to cell modification

58%
Witkowski K., Krol T., Lotocka M.
Oceanologia
|
1994
|
tom 36
|
nr 1
12

Adam Prażmowski, konstruktor pryzmatów polaryzacyjnych

58%
Gawel A.
Wszechświat
|
1957
|
nr 03
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