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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Protocol for acclimatization of in vitro cultured Potamogeton praelongus - aspect of plantlet size and type of substrate

100%
Prausova R., Kozelkova Z., Safarova L.
Acta Societatis Botanicorum Poloniae
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2015
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tom 84
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nr 1
The aim of the experiment was to determine suitable substrate type and optimal plant size for transfer of plantlets from in vitro to ex vitro under experimental outdoor conditions. Tests focused on the effect of substrate type (muddy and sandy) and starting size of plantlets gained through in vitro seed germination (0-3, 3.1-5,5.1-6, 6.1-10 cm) on plant growth. Three parameters (fresh weight, length, and the number of leaves) were compared to evaluate growth. Basic water parameters in experimental water tanks were regularly measured (pH, temperature, electrical conductivity, shadow intensity) and controlled to reach similar conditions to those in the natural habitat of this species. Overwintering was studied in a cellar with newly defined size categories (<6, 6.1-8, 8.1-10, 10.1-12, 12.1-15 cm). Both substrate type and starting size of plantlets significantly impacted growth. Plantlets grew better in the muddy substrate while a 100% success rate of rooting was gained with a starting size of 6.1-10 cm in both substrates. The biggest increase in fresh weight was observed with a starting size of 3.1-5 cm and 5.1-6 cm in both substrates. The greatest increase in fresh weight was observed in plants with a starting size of 3.1-5 cm in the muddy substrate (more than 95% increase). The best overwintering results were gained in the 6.1-8 cm size category.
2
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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In vitro culture as a powerful method for conserving Iranian ornamental geophytes

84%
Hesami M., Naderi R., Yoosefzadeh-Najafabadi M., Maleki M.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2018
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tom 99
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nr 1
3

Advances in regeneration and micropropagation

84%
Bach A.
Biotechnologia
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1996
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nr 1
4

Adventitious root formation of chokeberry (Aronia melanocarpa Elliot) influenced by the pH of medium

67%
Zatyko J. M., Molnar I.
Fruit Science Reports
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1990
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tom 17
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nr 1
5
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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In vitro flowering and micropropagation of Lisianthus (Eustoma grandiflorum) in response to plant growth regulators (NAA and BA)

67%
Kaviani B., Zamiraee F., Zanjani S. B., Tarang A., Torkashvand A. M.
Acta Scientiarum Polonorum. Hortorum Cultus
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2014
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tom 13
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nr 4
In vitro flowering and micropropagation are useful for plant breeding programs and commercial production of important ornamental plants. In vitro conditions including media components, kind, concentration and ratio of plant growth regulators and culture conditions significantly affect in vitro flowering and micropropagation. There is no any report dealing with the in vitro flowering of Lisianthus (Eustoma grandiflorum). Here, a protocol was developed for flowering and high frequency in vitro micropropagation of E. grandiflorum, an ornamental plant. Micropropagation is an effective tools for propagation of ornamental plants in large scale. The aim of the present study was to evaluate the effect of different concentrations of NAA and BA on micropropagation and flowering of Lisianthus, in vitro. Used culture medium was MS enriched with 0, 0.1, 0.2 and 2 mg L-1 of NAA and BA. In establishment process of explants, the most shoot length (2.07 cm per plant) was obtained on medium supplemented with 0.1 mg L-1 BA (without NAA). Maximum shoot number (5.80 per plant) was produced in medium containing 0.1 mg L-1 BA along with 0.2 mg L-1 NAA. Bud explants in culture media containing 0.2 mg L-1 NAA (without BA) and 0.1 mg L-1 NAA along with 2 mg L-1 BA produced maximum node number (3.20 per plant). The largest number of root (14.53 per plant) and root length (3.87 cm per plant) were produced on 0.2 mg L-1 NAA without BA, also 0.2 mg L-1 BA plus 0.2 mg L-1 NAA and 0.2 mg L-1 BA without NAA. Explants produced flower on medium containing 0.1 mg L-1 BA along with 0.1 mg L-1 NAA without transition of callus formation. Flower was produced from callus in medium containing 0.1 mg L-1 BA along with 2 mg L-1 NAA. Regenerated plants showed 98% survival in greenhouse during acclimatization. Acclimatized plants were morphologically similar to the mother plants.
6
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Adjustments to in vitro culture conditions and associated anomalies in plants

67%
Isah T.
Acta Biologica Cracoviensia. Series Botanica
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2015
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tom 57
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nr 2
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