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Research on stimulation of protoplast development in vitro

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Grzebelus E., Mackowska K., Szklarczyk M., Jopek M., Sniegowska-Swierk K., Oleszkiewicz T., Moranska E., Baranski R.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2015

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tom 96

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nr 1

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The analysis of mitochondrial and total DNA in sugar beet plants regenerated from protoplasts

86%

Sadoch Z., Majewska-Sawka A., Jazdzewska E.

Folia Morphologica

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1998

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tom 57

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nr 1

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Professor E. Pojnar’s pioneer studies on isolated protoplasts, their continuation and development. In memory of Professor Edward Pojnar (1919-2011)

72%

Pindel A., Wiszniewska A., Piwowarczyk B.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2012

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tom 93

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nr 2

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Plant protoplast technology: current status

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Davey M. R., Anthony P., Power J. B., Lowe K. C.

Acta Physiologiae Plantarum

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2005

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tom 27

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nr 1

Robust and reproducible protoplast-to-plant systems are crucial for underpinning genetic manipulation technology involving somatic hybridisation and transformation. Novel and effective approaches for maximising the efficiency of such protoplast cultures include supplementation of media with surfactants and artificial gas carriers, such as perfluorochemicals and haemoglobin. Physical parameters, particularly electrostimulation, also enhance the development of protoplasts and protoplast-derived cells in cullure. DNA uplake into protoplasts is now a routine and universally accepted procedure in plant biotechnology for introducing and evaluating both short-term (transient) and long-term (stable) expression of genes in cells and regenerated plants. Imporlantly, protoplast fusion overcomes pre- and post-zygotic sexual incompatibility barriers and generates novel germplasm through new nuclear-cytoplasmic combinations. In this respect, considerable progress has been made in generating somatic hybrid plants, particularly in citrus, brassicas and polato. Isolated protoplasts are also a unique single cell syslem for evaluating aspects of ultrastructure, genetics and physiology, with potential for the biosynthesis of novel secondary products, including commercially-important recombinant proteins (e.g. antibodies), and as systems in toxicity screening. Recent advances in protoplast technology have benefited from advances in animal and microbial cell culture, with interesting parallels existing between these systems. Further innovations will necessitate the strengthening of interdisciplinary links in these research fields and the requirement for continued dialogue and cooperation between workers with diverse but complementary skills.

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Protoplast regeneration into plants: do the cell walls play any role?

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Wisniewska E., Majewska-Sawka A.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2005

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tom 47

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nr 1

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Electroporated intact BY-2 tobacco culture cells as a model of transient expression study

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Koscianska E., Wypijewski K.

Acta Biochimica Polonica

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2001

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tom 48

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nr 3

Transfer of foreign genes into plant cells can be accomplished by several methods: agrobacterium-mediated, microinjection, biolistic particle bombardment and electro- poration. The last one is frequently used for transfection of plant protoplasts for transient gene expression. Electroporation is a simple procedure and allows transfecting a large number of cells at one time. Square wave-modulated porators are the most efficient for introducing expression cassettes into plant protoplasts. Based on a protocol developed by Wu & Feng (Plant Cell Reports, 1999, 18, 381-386), we optimized conditions for transfection of intact Nicotiana tabacum BY-2 cells using square wave-modulated electroporator. To simplify screening for transfected gene expression we used constructs with a GFP marker gene.

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Water and solute transport across the phospholipid bilayer of artificial and biological membranes

51%

Brahm J., Funder J., Gasbjerg P. K.

Biophysics of Membrane Transport

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1992

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tom 11

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nr 1

Ograniczanie wyników

Research on stimulation of protoplast development in vitro

The analysis of mitochondrial and total DNA in sugar beet plants regenerated from protoplasts

Professor E. Pojnar’s pioneer studies on isolated protoplasts, their continuation and development. In memory of Professor Edward Pojnar (1919-2011)

Plant protoplast technology: current status

Protoplast regeneration into plants: do the cell walls play any role?

Electroporated intact BY-2 tobacco culture cells as a model of transient expression study

Water and solute transport across the phospholipid bilayer of artificial and biological membranes