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Evaluation of the possibility of horizontal gene transfer and accumulation of transgenic DNA from the diet in the bodies of rats

100%

Kosieradzka I., Vasko V., Szwacka M., Przybysz A., Fiedorowicz S.

Journal of Animal and Feed Sciences

2010

tom 19

nr 2

307-315

The procedures for GMO safety tests include traceability of transgenic protein and transgenic DNA if the plant constitutes a component in the diet for an animal. This is due to the possibility of horizontal transfer of genes, accumulation of transgenic DNA in consumer’s organs, or induction of antibiotic resistance in gastrointestinal tract microflora. The last possibility is related to the use of marker genes in the process of transformation. In an in vivo experiment conducted on laboratory rats with the use of transgenic cucumbers expressing the pre-prothaumatin gene, the presence of transgenic DNA in the tissue of kidneys and liver was not detected. Resistance to neomycin of gastrointestinal tract microflora of the rats fed the GMO diet was not found, despite the use of marker genes (npt II) in the process of transformation of the investigated plants.

Production of tannase through submerged fermentation of tannin-containing plant extracts by Bacillus licheniformis KBR6

100%

Das M. P. K., Mondal K. C., Pati B. R.

Polish Journal of Microbiology

2006

tom 55

nr 4

Tannins are water-soluble polyphenolic compounds found in plants as secondary metabolites. The presence of these substances in the barks of eight different plants was initially examined and their crude extracts were used separately as a substrate for production of tannase through submerged fermentation by Bacillus licheniformis KBR6. Tannase production as well as biodégradation of the substrate reached a maximum within 15 to 18 h against crude tannin extract obtained from Anacardium occidentale. Among different concentrations of the crude tannin tested, 0.5% (w/v) induced maximum synthesis of enzyme. Tannase production was higher by almost two-fold in the presence of crude tannin compared to pure tannic acid used as a substrate. It seems that industrial production of tannase, using bark extract of A. occidentale can be a very simple and suitable alternative to presently used procedures.

Distribution of tannic acid degrading microorganisms in the soil and comparative study of tannase from two fungal strains

100%

Mondal K. C., Samanta S., Giri S., Pati B. R.

Acta Microbiologica Polonica

2001

tom 50

nr 1

Ograniczanie wyników

1 Acta Microbiologica Polonica

1 Journal of Animal and Feed Sciences

1 Polish Journal of Microbiology

2 Mondal K. C.

2 Pati B. R.

1 Das M. P. K.

1 Fiedorowicz S.

1 Giri S.

1 Kosieradzka I.

1 Przybysz A.

1 Samanta S.

1 Szwacka M.

1 Vasko V.

1 2010

1 2006

1 2001

Wyniki wyszukiwania

Evaluation of the possibility of horizontal gene transfer and accumulation of transgenic DNA from the diet in the bodies of rats

Production of tannase through submerged fermentation of tannin-containing plant extracts by Bacillus licheniformis KBR6

Distribution of tannic acid degrading microorganisms in the soil and comparative study of tannase from two fungal strains