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The rare earth elements are increasingly being used as trace supplements in different fields. In this study, subcellular distribution, the chemical forms and toxicity of cerium (Ce) were evaluated for Elodea canadensis. The effect of Ce (5–20 mg L⁻¹) applied for 7 days was assessed by measuring changes in the nutrient elements, photosynthetic pigments, malondialdehyde and antioxidant systems. Ce accumulation was greatest in the cell walls, followed by the organelles and the soluble fraction. Ce levels were higher in cellulose and pectin than in other biomacromolecules. The toxic effects caused by Ce were shown by a reduction in photosynthetic pigments, disruption of nutrient elements, and increases in MDA content. E. canadensis shows Ce-induced oxidative stress by modulating antioxidant enzymes, such as guaiacol peroxidase and catalase. Elevated Ce levels may represent a potential risk for aquatic ecosystems.
Essential oil of Artemisia annua L. was investigated to find out its toxicity and physiological aspects on the slug Agriolimax agrestis, in controlled conditions (8±1°C, 75±5 RH and 14:10 LD). The slugs received different concentrations of essential oil treated radish leaves in methanol, while the control received methanol alone. LC10, LC30, LC50 and LC90 values were estimated at 4.67, 5.3, 5.81, 7.25%, respectively. The effect of the essential oil on some important enzymatic components like; cytochrome P450 monnooxygenase, acid phosphatase, alkaline phosphatase, lipase, amylase and protease were significantly increased compared to the control. These results indicate that the plant Artemisia annua L. not only shows toxicity but also shows some irreversible effect on some important biochemical components and deserves further investigation.
The effects of lead salts (chloride, nitrate, acetate) on the elongation growth of three cereal species (maize, wheat, rye) under the same growth conditions were studied. It has been found that the most toxic lead compound for maize was acetate, while the most sensitive to lead chloride was wheat. It is suggested that in order to obtain comparable results in experiments on the effects of different heavy metals on plant growth, the same salts of metals should be used.
This study was designed to check whether intravaginal application of estradiol and progesterone on chosen days of early pregnancy affects the development and survival of porcine embryos. Crossbred gilts were assigned to control (C-land C-2) or experimental (T-l and T-2) groups. Systemic blood samples were collected in groups C-l and T-l on days 3-18 of pregnancy. Gilts of control groups were supplied on days 2-6 and 12-17 of pregnancy with intravaginal cocoa butter suppositories. Gilts of experimental groups were intravaginally supplied on days 2-6 of pregnancy with estradiol (0.2 mg) to improve uterine blood supply and on days 12-17 of pregnancy gilts were supplied with progesterone (0.25 mg) to stimulate the secretory function of the endometrium during the periimplantation period. Systemic concentration of progesterone was elevated on days 3-6 and of estradiol on days 4, 5 and 10 of pregnancy in the experimental groups in comparison with the control gilts. The weight of the ovary and number of corpora lutea were similar in the control and experimental groups, but the number of follicles over 3 mm in diameter was higher in the experimental groups than in control (P < 0.01) on day 30 of pregnancy. The mean ovulation rate, total number of embryos and rate of embryo survival on day 30 of pregnancy were similar in the control and experimental groups. However, the weight, crown-rump length and width of embryos were greater in the experimental groups than in control (P < 0.001). The results demonstrated that the intravaginal application of estradiol and progesterone on precisely chosen days of early pregnancy stimulated embryo development. This study demonstrated that the timing of hormone administration must comply with the physiological effect of estradiol and progesterone on uterine blood supply to ensure embryo development and survival.
Insect growth regulators generally have a selective effect on the target insects and have practically no apparent side effect on non-target organisms especially vertebrates. Hence, insect growth regulators could be a suitable choice to control pests in stored products. Ten-day-old larvae of Indian meal moth Plodia interpunctella (Hübner) were expressed to the juvenile hormone analogue pyriproxyfen in order to have an effect on growth, metamorphosis, reproduction, lipid and protein contents of ovaries. The larvae were treated by 0.02, 0.04, 0.08, 0.16, and 0.3 ppm of JHA in an artificial diet where controls received acetone alone. The results indicated significant differences in duration of growth, mean longevity of hatched adults, percentage of emerged normal adults, abnormal pupae, hatched larvae and mean oviposition ratein addition to the lipid and protein of ovaries compared to the controls. An inhibition concentration of fifty (IF50) for prevention of emerging adults was recorded 0.134 ppm. Pyriproxyfen caused significant defects in the legs and wings of some adults and sever morphological changes in the ovaries of emerged adults. The results showed that pyriproxyfen may be applied as an insecticide to decrease the damage caused by Indian meal moth on stored products. Pyriproxyfen can be used with low side effects to humans.
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