The purpose of the study was to determine inhibitory effect of calcium chelator; ethylene glycol-bis(2-aminoethyl- ether)-N,N,N’,N’-tetraacetic acid (EGTA) on flowering of a short-day (SD) plant Pharbitis nil. It was found that 20 mM solution of EGTA applied on cotyledons of 5-d-old A nil seedlings four hours before the start of 16-h-long induction night decreased the flowering response bye55 % compared to the control plants not treated with this Ca2 chelator. It also caused a very significant decrease of photosynthesis rate, transpiration rate and stomatal conductance both in light and darkness conditions. The results of this study confirm earlier hypothesis suggesting the effect of Ca2+ and its modulators on A nil flowering is due to their influence on the stomata.
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