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Differentiation of Listeria monocytogenes on the basis of hemolytic and phosphatidylinositol specific phospholipase C activity and by PCR method

100%

Kotlowski R., Kaczmarek M., Kur J., Rudnicka W.

Polish Journal of Food and Nutrition Sciences

1996

tom 05

nr 3

The possibility of differentiation of L.monocytogenes from other Listeria species on the basis of hemolytic activity, the production of phosphatidylinositol-specific phospholipase C (PI-PLC) and the polymerase chain reaction (PCR) for the amplification of a DNA fragment of listeriolysine O (hly A) gene was compared. The screening of Listeria colonies for PI-PLC activity allowed to distinguish the pathogenic for humans L.monocytogenes bacteria from the majority of non-pathogenic Listeria spp. The amplification of DNA from Listeria lysates with two primers selected in area of the hly A gene made possible the differentiation of L.monocytogenes from other Listeria species, including hemolytic L.ivanovii and L.seeligeri bacteria as well as hemolytic or PI-PLC positive L.innocua strains.

Ograniczanie wyników

1 Polish Journal of Food and Nutrition Sciences

1 Kaczmarek M.

1 Kotlowski R.

1 Kur J.

1 Rudnicka W.

1 1996

Wyniki wyszukiwania

Differentiation of Listeria monocytogenes on the basis of hemolytic and phosphatidylinositol specific phospholipase C activity and by PCR method