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The cambial activity and periodicity of secondary xylem and phloem formation have been less studied in tropical tree species than in temperate ones. Cambium activity is the only source of timber production. Studies on its activity and xylem formation can provide helpful data for dendrochronology and possible factors that control tree growth, the yield and quality of wood. Cinnamomum camphora is an important fast-growing tree for furniture and sculpture wood in South China. Its dynamics of cambial activity was not reported. During the growth season in 2011, five trees of Cinnamomum camphora plantation were monitored on the campus of Huazhong Agricultural University in central china (located on 113°41’ to 115°05’ E and 29°58’ to 31°22’ N). Sampling time was from 13, February 2011 to 13, February 2012. Some small stems of 15–20 cm length were selected to sample in the middle of 2–3 years old health branches in the central crown of these trees. The observational study found that the cambium of camphor trees was active from March to October and dormant from November to February. Onset of cambial cell division was observed in early March, one week after sprouting of new buds. The morphology of the cells and number of cell layers in the cambium zone varied throughout the year. In early March, cambium reactivated with the rising of temperature, the cell wall was relative thick. The number of cell layers increased from 2–3 in middle Feb. to 3–5. In June, the number of cell layers in cambium reached the maximum of 5–7 in a radial direction. In mid March, phloem cells began to differentiate, followed by xylem three weeks later. The number of cell layers in immature phloem and xylem increased dramatically before mid June and the increment of cell layers in xylem was almost fifth times of phloem in the differentiating process. The phloem cells mainly stopped dividing new cells in end of October, but xylem cells were still in the activity a week later. The annual activities of cambium was in accordance with the air mean temperature change in 2011–2012. The results showed that the cambial activity and formation of xylem and phloem were related to the environmental factors, especially the temperature.
The ontogeny and (ultra)structure of vascular tissue in Lupinus luteus L. root nodules were studied by light and transmission electron microscopy in juvenile nodule primordia up to the 11th day after inoculation. Vascular meristem originated from centripetally dedifferentiated root cortical parenchyma, endodermis and pericycle. The vascular trace was formed between bacteroid tissue initials and the root stele. In the trace's proximal part, cambial strands connecting the vascular trace and root cambium were formed. In the distal part, non-anastomosing vascular bundles started differentiating from the trace at the end of the juvenile stage. In lupine, the formative stage of the indeterminate root nodule vascular system was shown to be unique within the legumes.
Fungal invasion of Scots pine phloem and sapwood was investigated during a period of 15 weeks following attack by the pine shoot beetle, Tomicus piniperda (L.). The study was conducted in Mielec-Mościska, where the pine trees were heavily damaged by shot-feeding of T. piniperda. In order to determine the species richness and occurrence frequency of fungi associated with T. piniperda in temporal succession, living and trap trees infested by T. piniperda were used. Results revealed great diversity of fungi associated with T. piniperda, including 3758 cultures and 57 fungi species. The most important groups of fungi were the blue-stain fungi and molds, including mainly Penicillium, Trichoderma and Mucor genera. Among ophiostomatoid fungi, Ophiostoma minus and O. piceae were the dominant species. Occasionally isolated species were Leptographium lundbergii, L. procerum, L. wingfieldii, Graphium pycnocephalum and Graphium sp. ‘W’. Molds and pathogenic O. minus were the first invaders of both phloem and sapwood, however molds were more frequently isolated from phloem and sapwood at a depth of 5 mm. Ophiostoma piceae and L. lundbergii followed O. minus in the sapwood invasion. These species were successively replaced by L. wingfieldii, L. procerum and Graphium species in the later stages of fungal invasion in pine sapwood.
The development and (ultra)structure of vascular tissue was studied in Lupinus luteus L. mature root nodules 25 and 60 days after inoculation. In the proximal part of the nodule vascular trace, extensive growth took place to match the secondary growth of the root. Development of bacteroid and vascular tissues was correlated. The non-anastomosing nodule vascular bundles elongated and branched due to the activity of nodule lateral meristems, thus forming an extensive vascular network within deep lobes of bacteroid tissue. The vascular trace and vascular bundles differed mainly in the location and ultrastructure of transfer cells and parenchymatous cells, in which the inner membrane of the nuclear envelope formed tubular invaginations within the dense chromatin. Development of the vascular system in the collar root nodule of lupine differed from that of cylindrical indeterminate nodules.
Six seedling hypocotyl anatomical characters of sugar beet diploid lines and triploid hybrids were measured. Root yield and sugar content of these lines and hybrids were evaluated in replicated field trials. Some of the studied hypocotyl characters: the diameter of the central core, the diameter of parenchymatic cells outside the central core and the width of xylem, correlated negatively with sugar content and positively with root yield. This suggests that these parameters can be used in preliminary selection of sugar-beet breeding material. Introducing such criteria into the breeding process could speed up the selection and reduce the number of expensive field trials.
The paper examines the influence of industrial emissions of sulphur (IV) and nitrogen (IV) oxides on the percentage of stem anatomical characteristics of the autochthonous woody plant species undergrowth of Acer platanoides L. and Fraxinus excelsior L. in the southern industrial zone of the city of Dnipro (Ukraine). It is ascertained, that the ratio of the primary cortex share to the central cylinder share does not change in the stems of annual shoots of the both studied woody plant species when they are exposed to the influence of the toxic gases. However, there are significant changes in the ratio of shares of histological elements in the undergrowth stems of F. excelsior. The use of both absolute and relevant values of anatomic parameters of the plant vegetative organs is needed to analyse the woody plants resistance to technogenic pollution of the environment.
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