Wyniki wyszukiwania

1

Phenol degradation by bacteria. Part one: Isolation and characterization of bacterial strain

100%

Dluhy M., Rybarikova D., Bales V.

Polish Journal of Environmental Studies

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1996

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tom 05

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nr 2

An effective microorganism for phenol degradation process was selected and isolated from the natural environment using basic screening. The tested strain Alcaligenes xylosoxidans ssp. denitrificans was compared in vital activity experiments to the CCM strain Pseudomonas putida 3423 in order to obtain optimal biochemical parameters and to find out the right way of cultivation for the preparation of an inoculum. The efficiency of phenol degradation at higher concentrations is strongly influenced by microorganism age and, of course, by the initial substrate concentration. The time of biodegradation with the increase of initial phenol concentration was increased, but to the critical concentration was independent of it. The method of preparation of an inoculum with highly induced oxygenase system was found. A suitable composition of the broth medium for precultivation and cultivation of tested microorganisms has resulted from this work.

2

Characterization of catechol 2,3-dioxygenase from Planococcus sp. strain S5 induced by high phenol concentration

86%

Hupert-Kocurek K., Guzik U., Wojcieszynska D.

Acta Biochimica Polonica

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2012

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tom 59

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nr 3

This study aimed at characterization of a new catechol 2,3-dioxygenase isolated from a Gram-positive bacterium able to utilize phenol as the sole carbon and energy source. Planococcus sp. strain S5 grown on 1 or 2 mM phenol showed activity of both a catechol 1,2- and catechol 2,3-dioxygenase while at a higher concentrations of phenol only catechol 2,3-dioxygenase activity was observed. The enzyme was optimally active at 60°C and pH 8.0. Kinetic studies showed that the Km and Vmax of the enzyme were 42.70 µM and 329.96 mU, respectively. The catechol 2,3-dioxygenase showed the following relative meta-cleavage activities for various catechols tested: catechol (100%), 3-methylcatechol (13.67%), 4-methylcatechol (106.33%) and 4-chlorocatechol (203.80%). The high reactivity of this enzyme towards 4-chlorocatechol is different from that observed for other catechol 2,3-dioxygenases. Nucleotide sequencing and homology search revealed that the gene encoding the S5 catechol 2,3-dioxygenase shared the greatest homology with the known genes encoding isoenzymes from Gram-negative Pseudomonas strains.

3

The isolation of microorganisms capable of phenol degradation

86%

Przybulewska K., Wieczorek A., Nowak A., Pochrzaszcz M.

Polish Journal of Microbiology

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2006

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tom 55

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nr 1

The results of a study on the composition of microflora settling the pilot bio filter bed that purifies the exhausting gases from a cable factory's coil-wire varnishing division are presented in this study. The ability of isolated bacterial strains to biodegrade phenol was also evaluated using culture media of various compositions. Phenol was introduced into the medium at the following concentrations: 0.25, 0.5, 0.75 and 1 g x dm⁻³. In addition, air in desiccators, where microorganisms grew, was saturated with phenol. The isolated microorganisms were graded by the phenol decomposition rate using gas chromatography. The beds of biofilters utilized in industry appeared to be the source of microorganisms capable of degrading phenol. The most active were: Rhodococcus rhodochrous, Gordonia spud, Pseudomonas pudda. Their mixture showed higher degradation activity than the particular isolates. Isolated and identified bacteria metabolized phenol at high rate (about 14 to 42 g x m⁻³ x h⁻³).

4

FAME profiles in Pseudomonas vesicularis during catechol and phenol degradation in the presence of glucose as an additional carbon source

72%

Mrozik A., Piotrowska-Seget Z., Labuzek S.

Polish Journal of Microbiology

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2007

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tom 56

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nr 3

The aim of this study was to evaluate the impact of catechol and phenol added to culture media separately and with glucose as an additional, easily-degradable carbon source on fatty acid methyl ester (FAME) composition in Pseudomonas vesicularis. Simultaneously, the degradation rates of aromatic substrates used were investigated in single and binary substrate systems. Both catechol and phenol treatments caused changes in the distribution of tested groups of fatty acids. The most noticeable changes included an increase in degree of fatty acid saturation, the appearance of branched and disappearance of hydroxy fatty acids as compared to the control sample with glucose. Under catechol or phenol treatment sat/unsat ratio showed the values of 8.63 and 11.38, respectively, whereas in control cells it reached the value of 2.66. The high level of saturation comes from the high content of cyclopropane fatty acids in bacteria under exposure to aromatic substrates, regardless of the presence of glucose. In these treatments their content was more than 3-fold higher compared to the control. It has been demonstrated that glucose supplementation of culture media containing single aromatic substrate extended the degradation rates of catechol and phenol by P. vesicularis, caused an increase in number of cells but did not significantly change the fatty acid profiles in comparison with bacteria growing on catechol and phenol added to the media individually.

5

Phenol removal in packed bed reactor under denitrifying conditions

58%

Blaszczyk M., Tong T. T. S., Przytocka-Jusiak M., Suszek A.

Acta Microbiologica Polonica

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2002

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tom 51

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nr 1

6

Phenols degradation by Fenton reaction in the presence of chlorides and sulfates

58%

Siedlecka E. M., Stepnowski P.

Polish Journal of Environmental Studies

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2005

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tom 14

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nr 6

Since they often inhibit abundant bacteriological populations in municipal wastewater treatment plants, alternative treatment methods should be investigated – including advanced oxidation processes. This paper studies the degradation of three representatives of phenolic compounds using the Fenton system in the presence of chlorides and sulfates: phenol, 2-chlorophenol and 2-nitrophenol. The presence of anions influenced degradation rates of all studied compounds, which is attributed to the reduction in hydroxyl radical generation due to the formation of iron-complexes as well as the formation of much less reactive inorganic radicals. Differences between degradation rates of studied compounds and their degradation products in comparison to the rates observed for pure components show higher resistance of formed intermediates in comparison to phenol or 2-nitrophenol. Additionally, it was found that present anions influence further biodegradation of phenolic compounds but not similarly for all the compounds.

7

Phenol-degrading denitrifying bacteria in wastewater sediments

58%

Tong T. T. S., Blaszczyk M., Przytocka-Jusiak M., Mycielski R.

Acta Microbiologica Polonica

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1998

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tom 47

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nr 2

Ograniczanie wyników

2 Acta Microbiologica Polonica

2 Polish Journal of Environmental Studies

2 Polish Journal of Microbiology

1 Acta Biochimica Polonica

2 Blaszczyk M.

2 Przytocka-Jusiak M.

2 Tong T. T. S.

1 Bales V.

1 Dluhy M.

1 Guzik U.

1 Hupert-Kocurek K.

1 Labuzek S.

1 Mrozik A.

1 Mycielski R.

1 Nowak A.

1 Piotrowska-Seget Z.

1 Pochrzaszcz M.

1 Przybulewska K.

1 Rybarikova D.

1 Siedlecka E. M.

1 Stepnowski P.

1 Suszek A.

1 Wieczorek A.

1 Wojcieszynska D.

1 2012

1 2007

1 2006

1 2005

1 2002

1 1998