The biotransformation of phosphogypsum in cultures of sulfate-reducing bacteria (SRB) isolated from crude petroleum-refining wastewaters or purified using activated sludge method was studied. Selection was with the microcosms method on Postgate and minimal medium with different carbon sources, Emerson medium and petroleum-refining wastewaters. Highest hydrogen sulfide production, in excess of 500 mg/L, was observed in culture of microorganisms isolated from purified petroleum-refining wastewaters in Postgate medium with phenol as sole carbon source. 76% phenol reduction with simultaneous biotransformation of 2.7g phosphogypsum/L (1350 mg SO₄/L) was obtained. The results regarding post-culture sediment indicated 66% utilization of phosphogypsum introduced into the culture (5 g/L), which reflects the active biotransformation of phosphogypsum by the community selected from the wastewaters.
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