Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: peptide acceptor

Sortuj według:

Ogranicz wyniki do:

Activity of partially purified UDP-N-acetyl-alpha-D-galactosamine: polypeptide N-acetylgalactosaminyltransferase with different peptide acceptors

100%

Porowska H., Paszkiewicz-Gadek A., Gindzienski A.

Acta Biochimica Polonica

1999

tom 46

nr 2

As part of investigations on the role of the UDP-GalNAc-ribosome complex in the initial O-glycosylation of proteins, we have isolated from porcine gastric mucosa GalNAc-transferase, mucin and apomucin, and its three fractions containing carbohydrate in the amounts: I - 1.6%, II - 0.65% and III - 0.00% (wt/wt) of apomucin mass. Amino acid analysis showed that fractions I and II contained slightly higher amounts of serine and threonine as compared to native mucin and apomucin. The short peptide Pro-Thr-Ser-Ser-Pro-Ile-Ser-Thr was the most effectively glycosylated. Our apomucin preparations are also good acceptors of GalNAc and can be used for testing of O-glycosylation in vitro.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Gindzienski A.

1 Paszkiewicz-Gadek A.

1 Porowska H.

1 1999

Wyniki wyszukiwania

Activity of partially purified UDP-N-acetyl-alpha-D-galactosamine: polypeptide N-acetylgalactosaminyltransferase with different peptide acceptors