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1

Namnazanie parwowirusa psow w hodowli komorek na mikronosnikach

100%
Borowski A., Kimak I.
Medycyna Weterynaryjna
|
2000
|
tom 56
|
nr 10
645-647
2

Struktura parwowirusa psów

100%
Adaszek L., Zdanska D., Zietek J., Winiarczyk S.
Życie Weterynaryjne
|
2009
|
tom 84
|
nr 12
3

Szybka diagnostyka zakazen wirusem CPV-2 u psow

100%
Hulas C., Anusz K., Lesniewski S. L., Dobrzynski A.
Medycyna Weterynaryjna
|
1996
|
tom 52
|
nr 03
175-177
Clinical signs, haematology (leucopenia, lymphopenia, neutropenia) and results at necropsy may only suggest a disease suspected of a parvovirus etiology. Real proof of this kind infection is based on identification of canine parvovirus in faeces of a sick dog. Because of the common occurence of CPV-2 infections in dogs there is need to introduce a rapid test which would make it possible to perform a proper diagnosis immediately after the collection of the samples of faeces in hospital. In this paper the presence of CPV-2 was demonstrated by means of the test developed by On-Site Biotech (Uppsala, Sweden) on 41 of 50 stool samples with clinical signs suggesting parvovirus disease. Of 41 infected dogs 40 were leucopenic (leucocytes ≤ 4.5 G/L), lymphopenic (lymphocytes ≤ 1.3 G/L) and neutropenic (neutrophiles ≤ 4.0 G/L). Of nine dogs uninfected with CPV-2, none showed leucopenia, lymphopenia or neutropenia.
4

Ewolucja parwowirusów u zwierząt mięsożernych

84%
Siemionek J., Zaleska-Wawro M., Szweda W., Przywara K.
Medycyna Weterynaryjna
|
2017
|
tom 73
|
nr 09
Canine parvovirus (CPV) isolated as a new virus in dogs is endemic in this population. The application of vaccination has not prevented the spread of the disease. New antigenic variants have been isolated in different geographic regions. The article presents the evolution of CPV in the global carnivore population and the epidemiology of parvovirus infections. CPV is a very good model for understanding the sudden appearance of a disease through cross-species transmission.
5

Porownanie klasycznej metody PCR oraz Real-Time Sybr-Green HRM PCR w rozpoznawaniu zakazen parwowirusowych u psow

84%
Adaszek L., Twarog D., Zietek J., Wojciechowski M., Winiarczyk S.
Medycyna Weterynaryjna
|
2009
|
tom 65
|
nr 10
683-686
The aim of this study was to compare a standard PCR and Sybr-Green HRM PCR in the diagnosis of canine parvoviral infections. A total of 22 feces samples were collected from dogs suspected of parvovirosis. The entire DNA for standard PCR and Real-Time PCR was isolated from the feces. In both methods this same pair of primers that allow the amplification of a fragment of VP 2 gene with a length of 1278 bp were used. The specificity of the obtained PCR products in the classical method were established based on the results of sequencing 8 out of 22 DNA probes and based on the comparison of their sequences with a CPV VP2 FJ 222823 sequence taken from the GeneBank. The specificity of Real-Time PCR products were established based on the analysis of their melting curve. In both standard and Real-Time PCR CPV DNA was detected in all 22 feces samples. The length of the obtained products was 1190 bp. To obtain a positive result in Real-Time PCR it was required to increase the number of the cycles from 30 to 60. The Ct values were between 43-53, and the analysis of the melting curve revealed that the Tm of Real-Time PCR products ranged between 80.5-85°C. Despite the results of this study indicating that both of these techniques are specific, sensitive, and repeated methods for detection of the CPV DNA, to shorten the time of Real-Time PCR the application of appropriate primers is required, which enables the amplification of shorter fragments of the DNA than those obtained in our study.
6

Zastosowanie testu PLA do oceny namnazania wirusa nosowki, adenowirusa typu 1 oraz parwowirusa psow w hodowlach komorkowych

84%
Mizak B., Borowski A.
Medycyna Weterynaryjna
|
1998
|
tom 54
|
nr 11
753-756
The infection of dogs with distemper virus (CDV), adenovirus type 1 (CAV-1) and parvovirus (CPV) is still being diagnosed by practitioners, even in vaccinated dogs. Laboratory techniques used for the isolation and identification of these viruses are usually time consuming and often their accuracy is not satisfactory. The presented studies concentrated on the adaptation of PLA (Peroxidase Linked Assay) for the evaluation of CDV, CAV-1 and CPV replication in tissue cultures. Comparable studies on titration of viruses tested on the basis of cytopathic effects and results of PLA revealed that the titre of CDV and CPV evaluated by CPE was lower than calculated using PLA. The difference was equal to 0.6 log for CDV and 0.7 log CPV. These results confirm four to five-fold higher sensitivity of PLA. Titre of adenovirus type 1 measured by both methods did not demonstrate significant differences which can be explained by production of regular and characteristic CPE and rapid spread of cell destruction. Results of PLA revealed that the expression of viral antigens takes place between 18 and 24 hours after cell inoculation, whereas the first cytopathic effect was visible after 48-96 hours. The repeatability of results obtained by PLA was significantly higher with p<0.05 than titration of viruses on the bases of CPE in the case of CAV-1 and CDV. The presented results confirm the high sensitivity and specificity of PLA, which could be widely used in studies on CDV, CAV-1 and CPV.
7

Niektore nowe dane dotyczace wirusologii i zakaznych gabczastych encefalopatii

84%
Larski Z.
Medycyna Weterynaryjna
|
2003
|
tom 59
|
nr 02
95-99
8

Parwowiroza psow

84%
Rypula K., Ploneczka K.
Magazyn Weterynaryjny
|
2005
|
tom 14
|
nr 09
35-37
9

Parwowirus psów - cz. 1. Przyczyny i objawy

84%
Glema M.
Animal Expert
|
2020
|
nr 6
10

Zmiennosc antygenowa parwowirusa psow

84%
Mizak B.
Magazyn Weterynaryjny
|
1995
|
tom 04
|
nr 3
193-195
11
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Techniki molekularne w rozpoznawaniu parwowirozy psów

67%
Adaszek L., Twarog D., Winiarczyk S.
Życie Weterynaryjne
|
2009
|
tom 84
|
nr 05
12

Biologiczna i immunogenetyczna charakterystyka parwowirusa wyizolowanego z przypadkow zakaznego zapalenia zoladka i jelit psow

67%
Majer-Dziedzic B.
Rozprawy Naukowe. Akademia Rolnicza w Lublinie
|
2002
|
nr 260
1-158
13

Co nowego w chorobach zakaznych ukladu pokarmowego psow? Prawdy i mity na temat wariantu parwowirusa psow 2c [CPV-2c]

67%
Ploneczka-Janeczko K.
Magazyn Weterynaryjny
|
2010
|
tom 19
|
nr 04
328-330
14
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Patogeneza i diagnostyka parwowirozy psów oraz genotypowanie CPV-2

59%
Kowalczyk M., Skrzypek K., Jakubczak A.
Życie Weterynaryjne
|
2018
|
tom 93
|
nr 07
15

Parwowiroza psów - problem wciąż aktualny

59%
Wojcik A., Zietek J., Winiarczyk S.
Medycyna Weterynaryjna
|
2019
|
tom 75
|
nr 08
Parvoviral infections of dogs are still a clinical problem throughout the world. This is despite the development of prevention, increased awareness among the owners and increasingly effective treatment protocols due to the extremely high virulence of the virus. Parvovirosis is a disease caused by a virus of the Parvoviridae family in various variants: CPV-2, CPV-2a, CPV-2b and CPV-2c, all of which produce similar clinical signs, including acute hemorrhagic enteritis and myocarditis. It is one of the most important viral pathogens, with extremely high morbidity and mortality. This study is a compendium of current knowledge about parvoviral infections in dogs. It describes their aetiology, pathogenesis and factors predisposing to parvovirosis. Special emphasis is placed on the description of clinical signs and treatment of sick dogs. Various diagnostic methods that are necessary to make the final diagnosis of the disease are described. The recommendations of the WSAVA on prophylactic vaccination are also analyzed.
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