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The course of anti-Neospora antibody kinetics during two consecutive pregnancies has been evaluated in five chronically infected dairy cows. The blood samples of cows were collected monthly. Anti-Neospora antibodies in blood sera were detected by indirect ELISA (ID-VET, France). During whole period of the study cows remained seropositive; with S/P% values (iELISA) ranging from 94 to 214%. The antibody kinetics determined by iELISA showed a significant increase (P<0.0059) of specific IgG antibodies in the third trimester of both pregnancies. The monitored cows gave birth to 10 healthy calves (4 steers and 6 heifers). To confirm the occurrence of vertical transmission of Neospora from mothers to offspring in the herd, dam-daughter serology was performed. Anti-Neospora antibodies was found in 4 from 6 heifers (>6 months old). Study presents original data reporting on the very similar persistent pattern of anti-Neospora antibody levels during the third trimester of pregnancies in all five dairies. Based on the high seropositivity of female offspring, the reactivation of a latent infection of cows rather than a re-infection can be supposed.
The round goby, Neogobius melanostomus (Pallas, 1811) was introduced into southern Baltic waters (including the Vistula Lagoon) from the Black Sea and Sea of Azov in the early 1990s. This study describes the parasites of the round goby in its new environment. In 2004, 486 round goby specimens from the Vistula Lagoon were examined for parasites. The following taxa were identified: Dermocystidium sp.; Protozoa: Trichodina domerguei domerguei (Wallengren, 1897); Digenea: Cryptocotyle concavum (Creplin, 1825), Diplostomum spp., Tylodelphys clavata (Nordmann, 1831), Bunodera luciopercae (M¨uller, 1776); Cestoda: Bothriocephalus scorpii (M¨uller, 1776), Eubothrium crassum (Bloch, 1779), Paradilepis scolecina (Rudolphi, 1819), Proteocephalus filicollis (Rudolphi, 1802), P. gobiorum Dogel et Bychovsky, 1939, Proteocephalus sp.; Nematoda: Anguillicola crassus Kuwahara, Niimi et Itagaki, 1974, Camallanus truncatus (Rudolphi, 1814), Contracaecum spp., Cystidicoloides ephemeridarum (Linstow, 1872), Dichelyne minutus (Rudolphi, 1819), Hysterothylacium aduncum (Rudolphi, 1802); Acanthocephala: Echinorhynchus gadi M¨uller, 1776, Pomphorhynchus laevis (M¨uller, 1776). The parasites found were all local species, already reported from Poland. Except for Dermocystidium sp., C. concavum, P. gobiorum, and D. minutes, they have already been recorded in other fish species in the Vistula Lagoon. The prevalence and mean intensity of infection was low (18.3%; 4.0 indiv. – this value does not include ciliates). The most frequent parasites included H. aduncum (9.9%, 1.2 indiv.) and A. crassus (9.1%, 1.2 indiv.). In addition, Dermocystidium sp., B. luciopercae, E. crassum, P. scolecina, P. filicollis, C. truncatus and C. ephemeridarum are reported from the round goby for the first time. As the fish has only recently appeared in the Vistula Lagoon, its parasitic fauna has not yet developed to the full.
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