Wyniki wyszukiwania

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Cytotoxic and apoptotic effect of nanoclinoptilolite on canine osteosarcoma cell lines

100%

Ulutas P. A., Kiral F., Ulutas B., Asici G. S. E.

Journal of Veterinary Research

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2020

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tom 64

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nr 4

2

The inhibition of in vivo tumorigenesis of osteosarcoma [OS]-732 cells by antisense human osteopontin RNA

100%

Liu S. J., Zhang D.-Q., Sui X.-M., Zhang L., Cai Z.-W., Sun L.-Q., Liu Y.-J., Xue Y., Hu G.-F.

Cellular and Molecular Biology Letters

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2008

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tom 13

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nr 1

11-19

Osteopontin (OPN) is a secreted, non-collagenous, sialic acid-rich protein which functions by mediating cell-matrix interactions and cellular signaling via binding with integrins and CD44 receptors. An increasing number of studies have shown that OPN plays an important role in controlling cancer progression and metastasis. OPN was found to be expressed in many human cancer types, and in some cases, its over-expression was shown to be directly associated with poor patient prognosis. In vitro cancer cell line and animal model studies have clearly indicated that OPN can function in regulating the cell signaling that ultimately controls the oncogenic potential of various cancers. Previous studies in our laboratory demonstrated that OPN is highly expressed in human osteosarcoma (OS) cell line OS-732. In this study, we successfully reduced the tumorigenecity of OS-732 cells xenotransplanted into nude mice, using the antisense human OPN (hOPN) RNA expression vector.

3

Dual effect of 2-methoxyestradiol on cell cycle events in human osteosarcoma 143B cells

100%

Golebiewska J., Rozwadowski P., Spodnik J. H., Knap N., Wakabayashi T., Wozniak M.

Acta Biochimica Polonica

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2002

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tom 49

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nr 1

We have demonstrated for the first time that the steroid metabolite, 2-methoxy- estradiol (2-ME) is a powerful growth inhibitor of human osteosarcoma 143 B cell line by pleiotropic mechanisms involving cell cycle arrest at two different points and apoptosis. The ability of 2-ME to inhibit cell cycle at the respective points has been found concentration dependent. 1 uM 2-ME inhibited cell cycle at G1 phase while 10 uM. 2-ME caused G2/M cell cycle arrest. As a natural estrogen metabolite 2-ME is expected to perturb the stability of microtubules (MT) in vivo analogously to Taxol — the MT binding anticancer agent. Contrary to 2-ME, Taxol induced accumulation of osteosarcoma cells in G2/M phase of cell cycle only. The presented data strongly suggest two different mechanisms of cytotoxic action of 2-ME at the level of a single cell.

4

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Gene expression pattern in canine mammary osteosarcoma

80%

Pawlowski K., Majewska A., Szyszko K., Dolka I., Motyl T., Krol M.

Polish Journal of Veterinary Sciences

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2011

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tom 14

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nr 1

Canine mammary sarcomas are usually very aggressive and easily metastasize. Unfortunately the biology of this type of tumor is not well known because they are a very rare type of tumors. The aim of this study was to find differences in gene expression patterns in canine mammary osteosarcomas (malignant) versus osteomas (benign) using DNA microarrays. Our microarray experiment showed that 11 genes were up-regulated in osteosarcoma in comparison to osteoma whereas 36 genes were down-regulated. Among the up-regulated genes were: PDK1, EXT1, and EIF4H which are involved in AKT/PI3K and GLI/Hedgehog pathways. These genes play an important role in cell biology (cancer cell proliferation) and may be essential in osteosarcoma formation and development. Analyzing the down-regulated genes, the most interesting seemed to be HSPB8 and SEPP1. HSPB8 is a small heat shock protein that plays an important role in cell cycle regulation, apoptosis, and breast carcinogenesis. Also SEPP1 may play a role in carcinogenesis, as its down-regulation may induce oxidative stress possibly resulting in carcinogenesis. The preliminary results of the present study indicate that the up-regulation of three genes EXT1, EIF4H, and PDK1 may play an essential role in osteosarcoma formation, development and proliferation. In our opinion the cross-talk between GLI/Hedgehog and PI3K/AKT pathways may be a key factor to increase tumor proliferation and malignancy.

5

The construction of the eukaryotic expression plasmid pcDNA3.1-azurin and the increased apoptosis of U2OS cells transfected with it

80%

Ye Z., Peng H., Fang Y., Feng J., Yang D. S.

Cellular and Molecular Biology Letters

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2007

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tom 12

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nr 3

In our previous study, we demonstrated that azurin could selectively trigger apoptosis in human osteosarcoma cell line U2OS cells. However, the rate of apoptosis (35.8 ± 3.2%) is not very high, and azurin is too expensive to obtain readily. To solve these problems, we constructed a eukaryotic expression plasmid containing the azurin gene with an influenza virus haemagglutinin 9 peptide HA epitope tag, and transfected the recombinant plasmid pcDNA3.1(+)/azurin into U2OS cells. RT-PCR and Western blot analysis validated the successful transfection and the expression of the azurin-HA protein. Conspicuous apoptosis of the transfected cells was detected by flow cytometry (FCM) and the DNA ladder test. The apoptosis rate reached 64.3 ± 13.1%. The transcriptional levels of the Bax and p53 genes increased significantly in U2OS cells transfected with pcDNA3.1(+)/azurin, but the Bcl-2 mRNA level decreased. There was no difference in the levels of Bcl-xl mRNA and Survivin mRNA. We propose that the transfection of the recombinant plasmid pcDNA3.1(+)/azurin can significantly induce apoptosis in U2OS cells. This is closely associated with the up-regulation of the transcriptional level of the Bax and p53 genes, and the down-regulation of that of the Bcl-2 gene.

6

Postawić wszystko na jedną kartę - kostniakomięsak u psów i kotów

61%

Stilwell N.

Weterynaria po Dyplomie

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2020

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tom 21

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nr 1

7

Podstawy stomatologii weterynaryjnej. Cz. XIV. Złośliwe zmiany w jamie ustnej

61%

Bellows J.

Weterynaria po Dyplomie

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2019

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tom 20

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nr 6

Ograniczanie wyników

Cytotoxic and apoptotic effect of nanoclinoptilolite on canine osteosarcoma cell lines

The inhibition of in vivo tumorigenesis of osteosarcoma [OS]-732 cells by antisense human osteopontin RNA

Dual effect of 2-methoxyestradiol on cell cycle events in human osteosarcoma 143B cells

Gene expression pattern in canine mammary osteosarcoma

The construction of the eukaryotic expression plasmid pcDNA3.1-azurin and the increased apoptosis of U2OS cells transfected with it

Postawić wszystko na jedną kartę - kostniakomięsak u psów i kotów

Podstawy stomatologii weterynaryjnej. Cz. XIV. Złośliwe zmiany w jamie ustnej