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The aim of the experiment was to determine the RYR1 genotype and to analyse the distribution of osteochondrosis (OC) in wild boars from different regions of Lithuania. Hair and bone samples were collected from wild boars of various sexes and ages that had been shot during hunting season. Genomic DNA was extracted from hair roots. The RYR1 gene was analysed with the PCR-RFLP method, using specific primers and restriction enzymes Alw211 and Hin61. Only one C allele (q = 1.00) and CC genotype of the RYR1 gene were identified. Thus 138 genotyped wild boars were stress-resistant with both dominant alleles intact (no mutation). OC was measured by the cut surface of the distal femur and humerus. The prevalence of OC among the 34 wild boars tested was 20.6%. 5 animals (14.7%) had OC lesions in the hind legs and 2 animals (5.9%) had them in the front legs. OC affected leg joints more often in adult females (third-years and older), but this dependence was not significant (p > 0.05).
The possible role of coagulatory disorders in pathogenesis of Legg-Calve-Perthes disease (Lepo) and osteochondrosis (OC) was examined. A decrease in protein C level in dogs with LCPD (94.31±4.74%) in comparison with healthy dogs (95.8±6.35%) was observed. Moreover, in OC affected animals, the value varied between 92.25±2.5% and 94.33±5.5%. The mean plasma fibrinogen level in control group was 2.69±0.65 mg/mL, whereas in OC groups significantly higher values were found. Platelets number varied between individuals but was within normal range in all groups. Taking into account a decrease in protein C plasma level and an increase in fibrinogen concentration, the relationship between developmental diseases and coagulation disorders was revealed in dogs.
Several types of pathological bony overgrowth are known from various dinosaur taxa but, except for stress fractures, are rarely reported from appendicular elements. Herein we describe pathological manual and pedal phalanges of a camarasaurid sauropod (SMA 0002), which show features rarely recognised in non-avian dinosaurs. They include lateral osteophytes and smoothing of phalangeal articular surfaces, a deep pit, proximal enthesophytes in pedal unguals, distal overgrowth associated with a fracture, and a knob-like overgrowth lateral to the distal condyles of a pedal phalanx. Their causes were assessed by means of visual examination, CT scans, and bone histology, where possible. The lateral osteophytes are interpreted as symptoms of osteoarthritis. The ossified tendon insertions in the unguals are most probably the result of prolonged, heavy use of the pedal claws, possibly for scratch-digging. The distal overgrowth is interpreted to have developed due to changed stress regimes, and to be the cause for the fracture. The deep pit represents most likely a case of osteochondrosis, whereas the knob-like overgrowth likely represents a post-traumatic phenomenon not previously reported in dinosaurs. The study confirms that a rigorous assessment of pathologies can yield information about behaviour in long-extinct animals.
The monitoring of pig osteochondrosis (OC) in Lithuania began in 2001. 1,596 pigs (791 gilts and 805 castrates) of various breeds and with an average weight of 95 kg were tested. Housing and feeding conditions were the same for all pigs. The pigs were selected at a control fattening station, slaughtered, and OC was measured according to the methods applied in Sweden by the cut surface of distal femur and humerus. The severity of this disease was scored in elbow and knee joints on a 0-5 point scale. The occurrence of OC among all the tested pigs of various genotypes made up 47.4%. The study indicated that castrated males were more inclined to this disease than gilts (50.4% vs. 44.4%). The investigation data indicated that OC should be controlled in the course of selecting pigs raised in breeding centers of Lithuania. Significant differences were not found (P>0.1- 0.5) when analyzing the influence of OC on the fattening performance of Lithuanian White (purebred, bacon and meat types), Swedish Yorkshire and German Landrace pigs, However, the dependence of this defect on muscularity and other carcass traits of pigs was indicated. Lean meat percentage, lean loin area and ham weight of pigs having OC lesions of leg joints were all higher than that of pigs not having this defect, when backfat thickness was lower.
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