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1

Influence of reagent formulation on mRNA quantification by RT-PCR using imported external standard curves

100%
Farriol M., Orta X.
Acta Biochimica Polonica
|
2005
|
tom 52
|
nr 4
Use of an imported external standard curve is common in real-time quantitative RT-PCR. Two practical strategies for long-term experiments include importing a grand mean standard curve to all accumulated runs or using daily imported standard curves, fixing the slope at the beginning of the experiment and calibrating successive runs with curves generated from this imported slope, adding a single standard that registers the variation in the y-intercept. This study determines the influence that a change in reagent lots has on these two calibration approaches when determining mRNA copy numbers of the ornithine decarboxylase and porphobilinogen deaminase genes. Two sets of determinations were run with the use of lot A and lot B. A marked decrease in the crossing points (Cp) in the standards for both genes at all concentration levels was observed with the change in lots. A grand mean standard curve was generated for each gene and each set and comparisons between the sets were performed. Statistically significant differences were found with respect to the y-intercept but not the slope, suggesting that the change of reagent lot affected the detection sensitivity but not the efficiency of the reaction. The excellent correlation coefficients obtained for these curves for each gene were not achieved when overall data from both sets were combined to generate an overall grand mean standard curve. We conclude that when faced with a change of RT-PCR reagent lot that will affect the detection sensitivity of the method, samples should be calculated with either the daily imported standard curves or with the respective grand mean standard curve for each lot.
2

Changes in activity of ornithine decarboxylase in winter triticale caused by grain aphid feeding

86%
Sempruch C., Wojcicka A., Makosz M., Leszczynski B.
Acta Physiologiae Plantarum
|
2007
|
tom 29
|
nr 1 Suppl.
3

Molecular cloning of AZIN2 and its expression profiling in goose tissues and follicles

86%
Kang B., Deng T., Chen Z., Wang X., Yi Z., Jiang D.
Folia Biologica
|
2018
|
tom 66
|
nr 1
4
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Content available

Induction of ornithine decarboxylase in normal and protein kinase C - depleted human colon carcinoma cells

86%
Ostrowski J., Szczepiorkowski Z., Trzeciak L., Rochowska M., Skurzak H., Butruk E.
Journal of Physiology and Pharmacology
|
1992
|
tom 43
|
nr 4
To examine the role of protein kinase С (PKC) in induction of human colon adenocarcinoma cell line, DETA/W, by polypeptide growth- promoting factors, ornithine decarboxylase activity (ODC) and DNA synthesis were determined in cells depleted of PKC. PKC depletion was achieved by prolonged cultivation (more than 30 passages) with 10⁻⁶ M phorbol 12-myristate 13-acelate. Lack of PKC in studied cells was proved by measurements of PKC activity and immunoreactivity. Although ODC activities and DNA syntheses in PKC-depleted cells were decreased by about 40-50% compared to normal DETA/W cells, the percentage increase of these mitogen-responsive reactions was quantitatively similar in both cell sublines. These results raise the possibility that not all of the biological responses to growth factors are connected with the activation of calcium-dependent PKC.
5
Content available remote

Roles of cellular polyamines in mucosal healing in the gastrointestinal tract

72%
Gao J.-H., Guo L.-J., Huang Z.-Y., Rao J. N., Tang C.-W.
Journal of Physiology and Pharmacology
|
2013
|
tom 64
|
nr 6
6
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Role of polyamines in gastroprotection induced by epidermal growth factor

72%
Brzozowski T., Drozdowicz D., Majka J., Polonczyk-Pytko J., Konturek S. J.
Journal of Physiology and Pharmacology
|
1991
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tom 42
|
nr 2
Polyamines have been shown to stimulate cellular growth and differentiation, though their role in the prevention of acute gastric lesion induced by various noxious agents has been little studied. Epidermal growth factor (EGF) exhibits gastroprotective and ulcer healing properties due to its potent mitogenic and growth promoting action. This study was designed to compare the gastroprotective effects of spermine and EGF against gastric damage induced by absolute ethanol, acidified aspirin and stress and to determine the role of endogenous polyamines in EGF-induced gast- troprotection. Spermine and EGF significantly reduced the lesions induced by all three ulcerogens. Oral administration of spermine or subcutaneous infusion of EGF in 24 h fasted rats with chronic gastric fistula resulted in similar inhibition of gastric acid and pepsin secretion. Pretreatment with difluoromethylornithine (DFMO), an irreversible inhibitor of ornithine decarboxylase (ODC), a hey enzyme in the biosynthesis of polyamines, did not affect ethanol lesions, but reversed the protective effect EGF but not spermine, against ethanol. This finding indicates that polyamines mediate, at least in part, EGF-induced gastroprotection. In tests with oral administration of aminoguanidine that is known to suppress the activity of diamino-oxidase (DAO) and to inhibit the degradation of polyamines, EGF showed a markedly enhanced gastroprotective activity against ethanol damage. Since indomethacin failed to affect the gastroprotective effects of spermine and EOF and neither of these agents influenced the mucosal generation of PGE₂ in intact or injured gastric mucosa, we conclude that prostaglandins are not the major factors in spermine- and EGF-induced gastroprotection. This study demonstrates that polyamines are highly effective against gastric lesions induced by various ulcerogens and that they act as primary mediators of EGF-induced gastroprotection.
7

Androgen receptor and c-Myc transcription factors as putative partners in the in vivo cross-talk between androgen receptor-mediated and c-Met-mediated signalling pathways

72%
Dudkowska M., Jaworski T., Grzelakowska-Sztabert B., Manteuffel-Cymborowska M.
Acta Biochimica Polonica
|
2007
|
tom 54
|
nr 2
Cross-talk between two signal transduction pathways leads to a negative regulation of androgen-induced ornithine decarboxylase (ODC) gene expression in the mouse kidney. One pathway is triggered by testosterone via the intracellular androgen receptor, AR, and the other is induced by antifolate CB 3717 or folate via hepatocyte growth factor and its cell membrane receptor c-Met. Here we report the studies of the expression of AR and c-Myc transcription factors involved in ODC transactivation. Administration of CB 3717 or folate decreased the expression of AR. In contrast, testosterone did not modify AR mRNA content but augmented the AR protein. Furthermore, we demonstrate that administration of folate, but not testosterone, increases c-Myc transcript and protein level. We also document that activation of both examined pathways does not decrease the testosterone-induced AR protein level, but markedly increases c-Myc protein which is nearly 2-fold up-regulated compared to its level evoked solely by testosterone. We suspect that this pronounced increase of c-Myc protein might have functional consequences mirrored by down-regulated expression of AR target genes, among them ODC.
8
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Polyamines and cell migration

72%
McCormack S. A., Johnson L. R.
Journal of Physiology and Pharmacology
|
2001
|
tom 52
|
nr 3
9

Testosterone-androgen receptor-mediated signalling pathway does not modulate in vivo c-myc expression up-regulated in antifolate-folate-injured mouse kidney

72%
Dudkowska M., Czopek A., Stachurska A., Manteuffel-Cymborowska M., Grzelakowska-Sztabert B.
Cellular and Molecular Biology Letters
|
2002
|
tom 07
|
nr Suppl.
10
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Ornithine decarboxylase in large bowel mucosa: regulation by gastrin, secretin and EGF

72%
Johnson L. R., Wang P., Haddox K.
Journal of Physiology and Pharmacology
|
1992
|
tom 43
|
nr 1
Rats were fasted 48 h and then injected once with either saline, pentagastrin, EGF, secretin or combinations of secretin and pentagastrin or EGF. Another group of rats was fasted and refed. Animals were killed 4 h later and ODC assayed in mucosa of the cecum, proximal colon, and distal colon. EGF significantly increased ODC activity in all 3 tissues. Secretin had no effect by itself on ODC or ODC stimulated by EGF. Pentagastrin significantly increased ODC of the cecum, and secretin completely inhibited the effect of pentagastrin. Refeeding fasted rats significantly induced activity in all three tissues. Immunocytochemistry using a highly specific polyclonal ODC antibody showed that ODC was confined to the crypt cells of the proximal colon. Antibody dilution techniques demonstrated that gastrin, EGF and refeeding increased the level of enzyme in these cells. Refeeding in addition caused the appearance of enzyme in surface epithelial cells. These results showed that colonic mucosal ODC is present in proliferative cells and is regulated by the same peptides known to regulate growth in this tissue. Colonic mucosal ODC also responds the same way as it does in the oxyntic gland and small bowel mucosa.
11

Influence of temperature and abscisic and gibberellic acids on polyamine biosynthesis in European beech (Fagus sylvatica L.) seeds during dormancy breaking

58%
Krawiarz K., Szczotka Z.
Acta Biologica Cracoviensia. Series Botanica
|
2008
|
tom 50
|
nr 1
The effects of the exogenous growth regulators abscisic and gibberellic acids (ABA and GA3) on the activity of arginine (ADC) and ornithine decarboxylases (ODC) during dormancy breaking were studied in beech seed. During cold-stratification at 3°C, ADC and ODC activity increased rapidly starting from week 7 in embryo axes and week 8 in cotyledons. At 15°C, ADC activity was higher than ODC activity in embryo axes until week 7 and in cotyledons until week 8. Exogenous growth regulators clearly affected ADC and ODC activity. In embryo axes, ADC activity reached its maximum under the influence of GA3 between weeks 4 and 8. In the control variant (water temp. 3°C), enzymatic activity was moderately high, peaking in week 9 when a high proportion of seeds already showed germinability. In cotyledons the influence of GA3 on ADC activity was noticeable particularly during the first and last weeks. In the control variant the pattern of changes in the activity of this enzyme was similar but at a much lower level. ABA in both organs clearly inhibited ADC activity, but particularly at the end of the experiment. ODC activity in all variants of the experiment was higher in embryo axes than in cotyledons. The dynamics of change in ODC activity were similar to the changes in ADC activity in embryo axes and in cotyledons.
12

Changes in activity of ornithine decarboxylase in winter triticale seedlings stressed by grain aphid attack

58%
Sempruch C., Wojcicka A., Makosz M., Leszczynski B.
Zeszyty Problemowe Postępów Nauk Rolniczych
|
2008
|
tom 524
The aim of the present work was to determine an influence of grain aphid, Sitobion avenae F. feeding on ornithine decarboxylase (ODC) activity within tissues of winter triticale. Experiments were carried out on aerial parts and roots of seedlings of two triticale cultivars: Tornado - more susceptible to S. avenae and Witon - less susceptible. The obtained results showed that ODC activity in shoots of cv. Witon decreased during the first week of grain aphid feeding and increased after 2 weeks. However, in the roots of this cultivar the enzyme activity decreased after 48 h and increased after 2 weeks. When susceptible cv. Tornado was studied the grain aphid attack reduced the ODC activity after 24 h of feeding. Moreover, the grain aphid attack reduced the ODC activity during the first week and increased after 2 weeks in the shoots and roots of cv. Tornado. The importance of the ODC activity and polyamines in stress reaction and defense mechanisms of winter triticale to S. avenae is discussed.
13

Influence of putrescine on anthocyanin production in callus cultures of Daucus carota mediated through calcium ATPase

58%
Sudha G., Ravishankar G. A.
Acta Physiologiae Plantarum
|
2003
|
tom 25
|
nr 1
14

Involvement of protein kinase C in various cellular systems transducting ischemia evoked signal

58%
Zablocka B., Domanska-Janik K.
Acta Neurobiologiae Experimentalis
|
1993
|
tom 53
|
nr 1
15

Can arginase be a marker of the large bowel neoplasia?

58%
Konarska L., Kolasa T., Albrecht P., Regula A.
Acta Biochimica Polonica
|
1993
|
tom 40
|
nr 1
16

Difluoromethylornithine counteracts lesion-induced astrogliosis in rat hippocampus : enhancement of inhibitory effect by combined treatment with GM1 ganglioside

58%
Jeglinski W., Skup M., Zaremba M., Oderfeld-Nowak B.
Acta Neurobiologiae Experimentalis
|
1996
|
tom 56
|
nr 2
17

Up-regulation of spermidine-spermine N1-acetyltransferase [SSAT] expression is a part of proliferative but not anabolic response of mouse kidney

51%
Dudkowska M., Stachurska A., Grzelakowska-Sztabert B., Manteuffel-Cymborowska M.
Acta Biochimica Polonica
|
2002
|
tom 49
|
nr 4
A differential expression pattern of spermidine/spermine N1-acetyltransferase (SSAT), the enzyme critical to proper homeostasis of cellular polyamines, is reported in mouse kidney undergoing hyperplasia and hypertrophy. We have shown that SSAT activity and SSAT mRNA are significantly induced by antifolate CB 3717 and folate that evoke a drug-injury-dependent hyperplasia. In contrast, SSAT activity is down-regulated in the testosterone-induced hypertrophic kidney, while SSAT mRNA is positively controlled by this androgen. Catecholamine depletion evoked by reser- pine drastically decreases the folate-induced activity of «S-adenosylmethionine decar- boxylase (AdoMetDC), which limits polyamine biosynthesis, but has no effect on SSAT activity augmented by CB 3717. Our results document that the increased SSAT expression solely accompanies the proliferative response of mouse kidney, and sug­gest the importance of post-transcriptional regulation to the control of SSAT activity in both hyperplastic and hypertrophic experimental models.
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