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Multiplex-PCR assay for identification of Klebsiella pneumoniae isolates carrying the cps Loci for K1 and K2 capsule biosynthesis

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Gierczynski R., Jagielski M., Rastawicki W., Kaluzewski S.

Polish Journal of Microbiology

2007

tom 56

nr 3

Multiplex-PCR assay for identification of Klebsiella pneumoniae isolates carrying gene clusters for biosynthesis of capsular polysaccharide (CPS) types K1 and K2 was developed. Genes wzc and orflO of the cps cluster were applied as K1 and K2 specific markers respectively. The assay specificity was confirmed using 147 isolates of Klebsiella spp. including 77 K-antigen reference strains. The multiplex-PCR assay was found simple and cost-effective tool for identification of K. pneumoniae clinical isolates of K1 and K2 geno-serotypes.

Ograniczanie wyników

1 Polish Journal of Microbiology

1 Gierczynski R.

1 Jagielski M.

1 Kaluzewski S.

1 Rastawicki W.

1 2007

Wyniki wyszukiwania

Multiplex-PCR assay for identification of Klebsiella pneumoniae isolates carrying the cps Loci for K1 and K2 capsule biosynthesis