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Oligonucleotide synthesis using the 2-[levulinyloxymethyl]-5-nitrobenzoyl group for the 5'-position of nucleoside 3'-phosphoramidite derivatives

100%

Kamaike K., Takahashi H., Morohoshi K., Kataoka N., Kakinuma T., Ishido Y.

Acta Biochimica Polonica

1998

tom 45

nr 4

A comparative study on the utility of 2-(levulinyloxymethyl)-5-nitrobenzoyl (LMNBz) and 2-(levulinyloxymethyl)benzoyl (LMBz) protecting groups for the 5'-positions of nucleoside 3'-phosphoramidite derivatives in the oligonucleotide synthesis is presented in terms of the syntheses of TpTpT, TpTpTpT, and UpCpApGpUp-UpGpG. In addition we describe the synthesis, using the LMNBz protecting group, of the CpCpA terminus triplet of tRNAs bearing exocyclic amino groups with 15N-labeling, and the trimer Gp[A*]pG containing 2'-O-(β-D-ribofuranosyl)adenosine ([A*]), the latter of which is found at position 64 in the yeast initiator tRNAMet.

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Manipulation of vanillin synthesis genes in flax

63%

Pastor M., Boba A., Zuk M., Szopa-Skorkowski J.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

2015

tom 96

nr 1

Recent progress in oligonucleotide synthesis

63%

Pfleiderer W., Matysiak S., Bergmann F., Schnell R.

Acta Biochimica Polonica

1996

tom 43

nr 1

New blocking group combinations for the machine-aided oligoribonucleotide synthesis on solid phase material have been developed and tested regarding their general application. An acetal function for 2'-OH protection offers a series of advantages in the synthetic approach but special conditions have to be fullfilled in order to guarantee a selective cleavage of the temporary 5'-OH blocking group such as the dansylethoxycarbonyl or even the acid-labile dimethoxytrityl group in the chain elongation process. The final removal of the 2'-0-acetal function in the partially deblocked oligomer proceeds unexpectedly well under weak acidic conditions due to a supposed intramolecular acid catalysis.

Ograniczanie wyników

2 Acta Biochimica Polonica

1 BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

1 Bergmann F.

1 Boba A.

1 Ishido Y.

1 Kakinuma T.

1 Kamaike K.

1 Kataoka N.

1 Matysiak S.

1 Morohoshi K.

1 Pastor M.

1 Pfleiderer W.

1 Schnell R.

1 Szopa-Skorkowski J.

1 Takahashi H.

1 Zuk M.

1 2015

1 1998

1 1996

Wyniki wyszukiwania

Oligonucleotide synthesis using the 2-[levulinyloxymethyl]-5-nitrobenzoyl group for the 5'-position of nucleoside 3'-phosphoramidite derivatives

Manipulation of vanillin synthesis genes in flax

Recent progress in oligonucleotide synthesis