Wyniki wyszukiwania

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Expression profiles of toll-like receptors in the differentiation of an infection with Borrelia burgdorferi sensu lato spirochetes

100%

Dudek S., Ziolko E., Kimsa-Dudek M., Solarz K., Mazurek U., Wierzgon A., Kokot T., Muc-Wierzgon M.

Archivum Immunologiae et Therapiae Experimentalis

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2017

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tom 65

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nr 2

2

NBL 1 and anillin [ANLN] genes over-expression in pancreatic carcinoma

84%

Olakowski M., Tyszkiewicz T., Jarzab M., Krol R., Oczko-Wojciechowska M., Kowalska M., Kowal M., Gala G. M., Kajor M., Lange D., Chmielnik E., Gubala E., Lampe P., Jarzab B.

Folia Histochemica et Cytobiologica

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2009

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tom 47

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nr 2

249-255

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Gene expression profile analysis in laryngeal cancer by high-density oligonucleotide microanalysis

84%

Markowski J., Oczko-Wojciechowska M., Gierek T., Jarzab M., Paluch J., Kowalska M., Wygoda Z., Pfeifer A., Tyszkiewicz T., Jarzab B., Niedzielska I., Borgiel-Marek H.

Journal of Physiology and Pharmacology. Supplement

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2009

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tom 60

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nr 1

57-63

The assessment of gene expression profile in laryngeal cancer shall allow to implement molecular biology methods in diagnostics, as well as in prognosis of the course of disease. Thus, it may influence the choice of the most optimal decisions in regards to the method of treatment, extent of surgical procedure, or the necessity of adding post-operative radiotherapy. The aim of the project was to analyse the gene expression profile of laryngeal cancer using oligonucleotide microarrays, aiming to derive novel molecular markers for that carcinoma. The study comprised a group of 14 patients (12 males and 2 females) with squamous cell laryngeal carcinoma, diagnosed and surgically treated between 2005 – 2007 in the ENT Department of the Silesian Medical University in Katowice, Poland. RNA was isolated from frozen tissue fragments. To assess gene expression profile, high density oligonucleotide microarrays (Affymetrix U 133 Plus 2.0) were applied, with over 54 thousand probesets for over 47 thousand transcripts. Four genes, previously not assesed in diagnostic context in laryngeal carcinoma, seemed to be valuable markers of that neoplasm. These are: metalloproteinase ADAM12, cycline-dependent kinase 2 - CDK2, kinesine 14 - KIF14, suppressor 1 of checkpoint - CHES1.

4

MilkProtChip - a microarray of SNPs in candidate genes associated with milk protein biosynthesis - development and validation

67%

Kaminski S., Ahman A., Rusc A., Wojcik E., Malewski T.

Journal of Applied Genetics

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2005

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tom 46

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nr 1

MilkProtChip is an oligonucleotide microarray based on the arrayed primer extension (APEX) technique, allowing genotyping of single nucleotide polymorphisms (SNPs) in genes of interest for bovine milk protein biosynthesis. APEX consists of a sequencing reaction primed by an oligonucleotide anchored with its 5’end to a glass slide and terminating one nucleotide before the polymorphic site. The extension with one fluorescently labeled dideoxy nucleotide complementary to the template reveals the polymorphism. A total of 75 SNPs were selected among those associated directly or potentially with milk protein content. Among the 75 SNPs, 4 did not produce a positive signal. Most of the remaining SNPs produced a signal for both strands, except for 4 (one strand). In the validation step, 12 Polish Holstein bulls, 1 Polish Red bull, 1 bison (Bison bonasus), 11 Jersey cows and 25 Polish Holstein cows were screened to validate SNPs. Among the 71 selected SNPs - 26 were found monoallelic, the rest showing at least two genotypes for the entire population under study. All the animals were earlier genotyped for 2-5 SNPs by PCR-RFLP and PCR sequencing and all showed complete concordance with APEX genotyping. APEX reactions showed relatively high signal frequencies: more than 0.9, 0.9-0.8 and below 0.8, for 65,4 and 2 DNA samples, respectively. The primary application of the MilkProtChip is the simultaneous genotyping of dozens of SNPs to reveal and clarify the genetic background of milk protein biosynthesis. The chip may possibly be used for dairy cattle identification and paternity analysis, evolutionary studies, the evaluation of genetic distances between wild and domestic cattle breeds and the domestication history of bovine species.

Ograniczanie wyników

Expression profiles of toll-like receptors in the differentiation of an infection with Borrelia burgdorferi sensu lato spirochetes

NBL 1 and anillin [ANLN] genes over-expression in pancreatic carcinoma

Gene expression profile analysis in laryngeal cancer by high-density oligonucleotide microanalysis

MilkProtChip - a microarray of SNPs in candidate genes associated with milk protein biosynthesis - development and validation