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A 15-day experiment was performed on male Wistar rats allocated into three blocks diversified by a daily thiamine dose: 0, 20, 40 μg/day/rat. In each block the rats were divided into five groups depending on the type of diet: control group (FF) – fructan-free diet; experimental groups (I-5, I-10, OF-5, OF-10) – diets containing: 5%, 10% of inulin, as well as 5%, 10% of oligofructose respectively. Thiamine-free experimental diets were prepared according to AIN-93M recommendations, where inulin and oligofructose were added instead of wheat starch. The thiamine was administered per os as a water solution. Compared to the initial value, caecal pH decreased in all groups of rats. The daily thiamine dose as well as the kind of fructan influenced caecal pH. There could be observed the synergistic action of inulin and thiamine in decreasing pH. Inulin and oligofructose, due to prebiotic properties, can cause fluctuations in the caecum pH but the direction of changes is closely dependent on the presence of dietary thiamine. The most suitable pH values for endogenous thiamine uptake are reported during dietary deficit of this vitamin, independently on the type and dose of fructan.
Background. Fructans and chromium(III) are believed to influence lipid and carbohy-drate metabolism in animal and human organisms, that can be applied in regulation or improvement of lipid indices, however the importance of their effect is still unclear. This study aimed at evaluation of the effect of inulin-type fructans and chromium(III) supplementation on fat digestibility and blood lipid indices in rats. Material and methods. In a three-factorial design 56 male Wistar rats were fed ad libi-tum semipurified diets (AIN-93) supplemented with two types and levels of fructans (inulin or oligofructose, 5% or 10% of diet) and two levels of chromium(III) (0.5 or 5 mg/kg of diet; as Cr(III) propionate complex) for 10 weeks. Apparent fat digestibility and blood lipid profile were determined. Results. It was found that experimental factors did not influence apparent fat digestibility, serum total cholesterol, HDL and HDL cholesterol concentrations, however serum TAG level was significantly lower in animals fed oligofructose vs. inulin diets, high-fructan vs. low-fructan diet. Conclusions. Inulin type fructans and supplementary Cr(III) do not affect apparent fat digestibility, fructans however influence lipid metabolism, lowering serum TAG levels in rat
The aim of the study was to estimate the effect of 5% and/or 10 % of inulin and/or oligofructose on thiamine level in blood serum and urine. The vitamin was administered in doses of: 20 or 40 μg/day/rat. With respect to the control (fructan-free), the diet containing 5% of inulin decreased (p<0.05) serum thiamine level when the lower daily vitamin dose was used, and oligofructose had no influence. It was observed that all experimental diets decreased urine thiamine level (p<0.05), which remained stable until the 9th day of the experiment. Then, after next 3 days, the urine thiamine increased, except for the I-5 group with 20 μg daily thiamine dose. The diet with 5% of oligofructose increased (p<0.05) average daily thiamine excretion when rats consumed 20 μg of vitamin per day. Oligofructose was demonstrated to be capable of improving thiamine nutritional status in thiamine-malnourished rats.
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