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The aim of the paper was to identify the influence of magnesium on the preservation of pathogenic abilities of entomopathogenic nematodes living in soil contaminated with oil derivatives. Entomopathogenic nematodes living under natural conditions in soil or applied to soil as biopreparations for plant pest control are sensitive to soil contamination with heavy metals and oil derivatives. These nematodes do not respond directly by higher mortality but by a decrease in their pathogenic abilities, which adversely affects the success in searching and eliminating pests. A Polish commercial preparation called Owinema SC, containing infective juveniles of Steinernema feltiae Filipjev (Rhabditida: Steinernematidae), was used to test the effect of oil derivatives on pathogenicity and reproduction of entomopathogenic nematodes. Soil was polluted with petrol, diesel oil and used engine oil; the control was soil unpolluted with oil derivatives. Magnesium sulphate was also added (MgSO4∙H2O) in the amount of 160 mg per 1 liter of the suspension. The three oil derivatives in concentrations of: 0.3, 0.6 and 1.0 g or in double doses, i.e. 0.6, 1.2 and 2.0 g were poured over weighted soil batches. The control remained intact. Three replications of each treatment were made. Subsequently, Tenebrio molitor L. larvae used as bait inesct were placed in containers with the suspension. The bait insects were kept in the containers for 7 days, and afterwards they were removed from the soil and taken from the traps in order to check their mortality rate. Furthermore, to test the reproduction ability of entomopathogenic nematodes, dead larvae were moved to “islands” previously prepared according to the Dutky method. The results were presented as the number of entomopathogenic nematodes per 1 dead larvae of Tenebrio molitor L.
The study concerned PCB accumulation in soil at two depths (S1: 0-20 cm, S2: 20- 40 cm) and in Taraxacum officinale plants. It was carried out within railway junctions and near railway lines. Various degrees of PCB contamination were detected in soil and parts of plants above the ground level. PCB content in most soil samples was between 100 and 250 ng/g, while in plants it varied from 100 to 800 ng/g. An analysis of the distribution of different groups of PCB congeners in plant and soil material has revealed that the content of hepta-CB and hexa-CB fractions was the highest of all PCBs for both soil depths, as well as for plants. No significant differences were found for the degree of various PCB congener group penetration into the soil, neither in railway junction areas, nor in the vicinity of railway tracks. Statistical test of pair comparison, performed in order to establish the rate at which plants accumulate different groups of PCB congeners has revealed significant differences in accumulation rates for the following pairs: penta-chlorinated congeners are accumulated at a higher rate than tetra-chlorinated ones and hexa-chlorinated congeners are accumulated at a higher rate than penta-chlorinated ones. This phenomenon occurs in the area of railway junctions (areas heavily polluted with oil derivatives). It was found that in the area of railway lines (areas with low levels of pollution) hexa-chlorinated congeners were accumulated at a higher rate than tetra- and penta-chlorinated ones. No significant differences were found for other pairs of PCB congeners. The use of dandelion as a bioindicator of environmental pollution with PCB congeners seems to be a good and reliable source of information about the emission of those substances into the natural environment.
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