The observations were done on 10 bulls at the age of 3—4 years in two experimental groups. Group A — 5 bulls with the coital exanthema and group В — 5 healthy bulls. Clinical, bacteriological, virological (serology, immunofluorescence, cultivation) and immunological examinations were performed on days 1, 14, 28, 58 and 84. Serological examinations included a spontaneous and stimulated leukocyte migration and PMN’s migration, number of PMN’s, phagocytic activity of PMN, the NBT and the test of intracellular killing. Moreover, there was examined a specific cellular immunity by the skin test (DTH) against a specific antigen, by migration inhibition test of leukocytes (LIMF) and granulocytes (LIF). The presence of specific antibodies against PI-3, BHV-1, VDMD, RSV, adeno-2 and 5, BLV was determined by HI, SN, CF, immunodiffusion and ELISA. Moreover culture studies and the direct immunofluorescence test were used to detect BHV-1. Cultural studies to detect aerobic and anaerobic bacteria, mycoplasms, ureaplasms and sero-cultural studies to exclude chlamydial infections were performed. In bulls with the coital exanthema desides changes in reactivity of lymphocytes В and T a profoud changes concerned also the phagocytic activity of PMN’s and/or leukocytes confirming the role of unspecific cellular immunity in bulls with coital exanthema.
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