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The present study was undertaken to evaluate the effects of divercin, a bacteriocin produced by lactobacilli strain Carnobacterium divergens AS7, on the microflora status under in vitro conditions and on nutrient retention and nitrogen-corrected apparent metabolizable energy (AMEN ) of divercin in an in vivo trial on broiler chickens. Low (DL) 200 AU·ml1 (0.05% of the liquid divercin prepatation), and high (DH) 1600 AU·ml-1(0.4% of the liquid divercin prepatation) doses of divercin were used in both trials. In the in vitro trial divercin at concentration, 1600AU ml-1 of divercin had stronger antibacterial effects as compared with 200 AU·ml-1. In the crop and ileal digesta, the DH treatment was characterized by the lowest lactic acid bacteria (LAB) and coliform bacteria counts (0.4-0.8 log cycle reduction). There were no differences in nutrient retention between treatments. Salinomycin and divercin supplementation tended to increase fat digestibility and N retention. However, the highest AMEN were obtained in the DL treatment. The results of both studies show positive effects of divercin in terms of reduction of microbial populations isolated from the gastrointestinal tract (GIT) of broiler chickens as well as improvement in AMEN. The presented data may suggest that bacteriocin derived from Carnobacterium divergens AS7 could play a role in controlling the microbial ecosystem in the broiler chicken GIT.
In a mosaic landscape moderately affected by agriculture and tourism, such as found in the Masurian Lakeland (north-eastern Poland) external input of nutrients (TP, TN, NNO3) and their retention were compared in a cascade of shallow in-river lakes, between several years of different weather conditions. The average and maximum air temperature in the summer and winter months and that of the freshet (April) tended to be higher in the late 90s than the late 70s. The annual summer precipitation was lower in some years of the 90s than the 70s. This combination of dry and warm conditions (especially in summer) resulted in the water discharge, renewal rate of lake water and nutrient loads to the lakes becoming drastically reduced and more differentiated between freshet and other periods. This imbalance resulted in the occasional higher concentration of nutrients (due to internal loading) in lake outflows and lower retention of nutrients in the lake systems. Downstream exports of TP or TN took place occasionally from the lakes, although N-NO3 was usually retained. Speculations are made about how frequently this situation may affect surface-fed, shallow lakes in the light of global warming (already detected in the region) and how important this could become for future eutrophication in shallow, temperate lakes generally.
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