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Degradation of extracellular nucleotides and their analogs in HeLa and HUVEC cell cultures

100%

Gendaszewska-Darmach E., Maszewska M., Zaklos M., Koziolkiewicz M.

Acta Biochimica Polonica

2003

tom 50

nr 4

The use of nucleotides and their analogs in the pharmacological studies of nucleotide receptors (P2 class) should be preceded by detailed studies on their degradation connected with ecto-enzymes of a given cell type. In the present studies we have analyzed stability of some phosphorothioate and phosphonate analogs of ATP and ADP in the HeLa epitheloid carcinoma and endothelial HUVEC cells cultures. Our studies have revealed that ecto-nucleotide pyrophosphatase (E-NPP) is one of the main enzymes involved in the extracellular degradation of ATP and other nucleotides in the HeLa cells. On the other hand, the ecto-ATPDase is responsible for the hydrolysis of extracellular nucleotides in human endothelial cell cultures, while the E-NPP-like enzymes of the HUVEC cells are not essential to this degradation. The concerted action of the aforementioned ecto-enzymes and nucleotide pyrophosphatase, 5 -nucleo- tidase and adenosine deaminase present in fetal bovine serum (FBS) supplied to the culture medium, results in partial or complete degradation of the phosphorothioate (ATPγS) and phosphonate analogs of adenosine nucleotides (α,β-methylene-ATP and β,γ-methylene-ATP) in the cell cultures. Only ADPβS appears to be resistant to these enzymes. The influence of some nucleotides and their analogs on the proliferation of the HeLa cells in presence or absence of FBS is also discussed.

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Nucleotide analogue, method of synthesis of nucleotide analogue, use of nucleotide analogue, antiviral pro-nucleotide, pharmaceutical composition

84%

Kraszewski A., Romanowska J., Szymanska-Michalak A., Sobkowski M., Boryski J., Stawinski J., Lipniacki A., Piasek A.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

2014

tom 95

nr 4

Antibodies and nucleotide analogues: their effect on the allosteric response in the mammalian carbamoyl phosphate synthetase II [CPS II], the key reaction in pyrimidine biosynthesis

84%

Carrey E. A., Zhu L.

Cellular and Molecular Biology Letters

1999

tom 04

nr 3

Ograniczanie wyników

1 Acta Biochimica Polonica

1 BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

1 Cellular and Molecular Biology Letters

1 Boryski J.

1 Carrey E. A.

1 Gendaszewska-Darmach E.

1 Koziolkiewicz M.

1 Kraszewski A.

1 Lipniacki A.

1 Maszewska M.

1 Piasek A.

1 Romanowska J.

1 Sobkowski M.

1 Stawinski J.

1 Szymanska-Michalak A.

1 Zaklos M.

1 Zhu L.

1 2014

1 2003

1 1999

Wyniki wyszukiwania

Degradation of extracellular nucleotides and their analogs in HeLa and HUVEC cell cultures

Nucleotide analogue, method of synthesis of nucleotide analogue, use of nucleotide analogue, antiviral pro-nucleotide, pharmaceutical composition

Antibodies and nucleotide analogues: their effect on the allosteric response in the mammalian carbamoyl phosphate synthetase II [CPS II], the key reaction in pyrimidine biosynthesis