Wyniki wyszukiwania

1

Modified tRNAs for probing tRNA binding sites on the ribosome

100%

Podkowinski J., Dymarek-Babs T., Gornicki P.

Acta Biochimica Polonica

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1989

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tom 36

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nr 3-4

2

Modeling studies of potato nucleoside triphosphate diphosphohydrolase NTPDase1: an insight into the catalytic mechanism

100%

Kozakiewicz A., Neumann P., Banach M., Komoszynski M., Wojtczak A.

Acta Biochimica Polonica

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2008

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tom 55

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nr 1

Nucleoside triphosphate diphosphohydrolase - NTPDase1 (apyrase, EC 3.6.1.5) was modeled based on sequence homology. The single polypeptide chain of apyrase is folded into two domains. The putative catalytic site with the apyrase conserved regions (ACR 1-5) is located between these two domains. Modeling confirmed that apyrase belongs to the actin superfamily of proteins. The amino acids interacting with the nucleoside triphosphate substrate and probably involved in the catalyzed hydrolysis were identified. The proposed two-step catalytic mechanism of hydrolysis involves Thr127 and Thr55 as potential nucleophilic factors responsible for the cleavage of the Pγ and Pβ anhydride bonds, respectively. Their action seems to be assisted by Glu170 and Glu78 residues, respectively. The presence of two nucleophiles in the active site of apyrase explains the differences in the hydrolytic activity between apyrases and other enzymes belonging to the NTPDase family.

3

Different responses for Ap5A and ATP in human cerebrocortical synaptic terminals

100%

Pintor J., Diaz-Hernandez M., Gualix J., Gomez-Villafuertes R., Miras-Portugal T.

Cellular and Molecular Biology Letters

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1999

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tom 04

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nr 3

4

In search of ionizing radiation effect on nucleosides: radiolysis of cytidine

84%

Zavyalova O., Janiak A., Misiura K., Truszkowski S., Chostenko A.

Current Topics in Biophysics. Supplement

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2011

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tom 34

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nr A: Posters

5

Cellular level of 8-oxo-2'-deoxyguanosine in DNA does not correlate with urinary excretion of the modified base nucleoside

84%

Foksinski M., Gackowski D., Rozalski R., Olinski R.

Acta Biochimica Polonica

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2003

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tom 50

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nr 2

We assessed a relationship between the level of 8-oxodG in leukocyte DNA measured with the high performance liquid chromatography with electrochemical detection (HPLC/EC) technique and urinary excretion of the modified nucleoside/base analysed with a recently developed methodology involving HPLC prepurification followed by gas chromatography with isotope dilution mass spectrometric detection. No correlation was found between these markers of oxidative DNA damage commonly used in epidemiological studies. Several possible explanations of this finding are discussed.

6

Guanine and inosine nucleotides, nucleosides and oxypurines in snail muscles as potential biomarkers of fluoride toxicity

84%

Rac M. E., Safranow K., Dolegowska B., Machoy Z.

Folia Biologica

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2007

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tom 55

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nr 3-4

7

Changes in membrane phospholipid biosynthesis induced by chloropromasine and nucleoside analogues: its therapeutic consequences

84%

Staub M., Sumeg R., Sasvari-Szekely M., Simmonds H. A.

Cellular and Molecular Biology Letters

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1999

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tom 04

|

nr 3

8

Raised plasma adenosine in chronic fatigue syndrome

84%

Duley J. A., Shobowale-Bakre E. M., Garrick P., Pratt D.

Cellular and Molecular Biology Letters

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1999

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tom 04

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nr 3

9

Adenosine as a metabolic regulator of tissue function: production of adenosine by cytoplasmic 5'-nucleotidases

84%

Borowiec A., Lechward K., Tkacz-Stachowska K., Skladanowski A. C.

Acta Biochimica Polonica

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2006

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tom 53

|

nr 2

Adenosine is a product of complete dephosphorylation of adenine nucleotides which takes place in various compartments of the cell. This nucleoside is a significant signal molecule engaged in regulation of physiology and modulation of the function of numerous cell types (i.e. neurons, platelets, neutrophils, mast cells and smooth muscle cells in bronchi and vasculature, myocytes etc.). As part a of purinergic signaling system, adenosine mediates neurotransmission, conduction, secretion, vasodilation, proliferation and cell death. Most of the effects of adenosine help to protect cells and tissues during stress conditions such as ischemia or anoxia. Adenosine receptors and nucleoside transporters are targets for potential drugs in many pathophysiological situations. The adenosine-producing system in vertebrates involves a cascade dephosphorylating ATP and ending with 5'-nucleotidase (EC 3.1.3.5) localized either on the membrane or inside the cell. In this paper the cytoplasmic variants of 5'-nucleotidase are broadly characterized as well as their clinical relevance. The role of AMP-selective 5'-nucleotidase (cN-I) in the heart, skeletal muscle and brain is highlighted. cN-I action is crucial during ischemia and important for the efficacy of some nucleoside-based drugs and in the regulation of the substrate pool for nucleic acids synthesis. Inhibitors used in studying the roles of cytoplasmic and membrane-bound 5'-nucleotidases are also described.

10

The phosphorylation of nucleoside analogs by deoxyribonucleoside kinases

84%

Karlsson A., Zhu C., Johansson M.

Cellular and Molecular Biology Letters

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1999

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tom 04

|

nr 3

11

Recent advances in understanding the relationship between adenosine metabolism and the function of T and B lymphocytes in diabetes

67%

Sakowicz-Burkiewicz M., Pawelczyk T.

Journal of Physiology and Pharmacology

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2011

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tom 62

|

nr 5

12

Some kinetic studies on cytidine aminohydrolase activity from Aspergillus niger NRRL3

67%

Ali T. H.

Acta Microbiologica Polonica

|

1998

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tom 47

|

nr 4

Ograniczanie wyników

Modified tRNAs for probing tRNA binding sites on the ribosome

Modeling studies of potato nucleoside triphosphate diphosphohydrolase NTPDase1: an insight into the catalytic mechanism

Different responses for Ap5A and ATP in human cerebrocortical synaptic terminals

In search of ionizing radiation effect on nucleosides: radiolysis of cytidine

Cellular level of 8-oxo-2'-deoxyguanosine in DNA does not correlate with urinary excretion of the modified base nucleoside

Guanine and inosine nucleotides, nucleosides and oxypurines in snail muscles as potential biomarkers of fluoride toxicity

Changes in membrane phospholipid biosynthesis induced by chloropromasine and nucleoside analogues: its therapeutic consequences

Raised plasma adenosine in chronic fatigue syndrome

Adenosine as a metabolic regulator of tissue function: production of adenosine by cytoplasmic 5'-nucleotidases

The phosphorylation of nucleoside analogs by deoxyribonucleoside kinases

Recent advances in understanding the relationship between adenosine metabolism and the function of T and B lymphocytes in diabetes

Some kinetic studies on cytidine aminohydrolase activity from Aspergillus niger NRRL3